Hyperpolarization-activated cationic currents (Ih) in neurones of the trigeminal mesencephalic nucleus of the rat

The Journal of Physiology
B S Khakh, G Henderson

Abstract

1. We studied the voltage-dependent current activated by membrane hyperpolarization in sensory proprioceptive trigeminal mesencephalic nucleus (MNV) neurones. 2. Membrane hyperpolarization (from -62 to -132 mV in 10 mV steps) activated slowly activating and non-inactivating inward currents. The hyperpolarization-activated currents could be described by activation curves with a half-maximal activation potential (V ) of -93 mV, slope (k) of 8.4 mV, and maximally activated currents (Imax) of around 1 nA. The reversal potential of the hyperpolarization-activated currents was -57 mV. 3. Extracellular Cs+ blocked hyperpolarization-activated currents rapidly and reversibly in a concentration-dependent manner with an IC50 of 100 microM and Hill slope of 0.8. ZD7288 (1 microM; 4-(N-ethyl-N-phenylamino)-1,2-dimethyl-6-(methylamino) pyridinium chloride), the compound developed as an inhibitor of the cardiac hyperpolarization-activated current (If), also blocked the hyperpolarization-activated currents in MNV neurones. Extracellular Ba2+ (1 mM) did not affect hyperpolarization-activated currents. We tested whether the hyperpolarization-activated currents contribute to the somatic membrane properties of MNV neurones by performing some exper...Continue Reading

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Citations

Aug 10, 2006·Anatomy and Embryology·J LierschK Zilles
Mar 12, 2009·Journal of the Association for Research in Otolaryngology : JARO·Ramazan Bal, Giyasettin Baydas
Mar 30, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Sebastian CurtiAlberto E Pereda
Mar 11, 2017·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Jin-Eon HanIl-Sung Jang
Mar 3, 2004·The Journal of Comparative Neurology·Takuya Notomi, Ryuichi Shigemoto
Mar 11, 2000·The European Journal of Neuroscience·S L MironovD W Richter
Aug 3, 2007·Journal of Neuroscience Research·Chie-Fang HsiaoScott H Chandler
Nov 26, 2005·The Journal of Physiology·Stéphanie ChevallierJean-Marie Cabelguen
Mar 4, 2003·Journal of Neurophysiology·Susumu TanakaScott H Chandler
Sep 14, 1999·Journal of Neurophysiology·C M PedroarenaF R Morales
May 8, 2008·Neurochemical Research·Yu-Qiu JiangYou Wan

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