PMID: 7541461Apr 1, 1995Paper

Hyperpolarization induced by vasoactive substances in intact guinea-pig endocardial endothelial cells

The Journal of Physiology
K ManabeA Noma

Abstract

1. The responses of guinea-pig endocardial endothelial (EE) cells to various vasoactive substances were investigated in either the small tissue preparation or freshly isolated cells using the patch clamp technique. 2. The mean resting potential of the EE cell was -44 mV in the small tissue preparation, and applications of ATP, ADP, AMP, adenosine, histamine and substance P induced transient hyperpolarizations of -22, -21, -9, -10, -23 and -15 mV, respectively. The membrane potential of EE cells failed to respond to acetylcholine, bradykinin, thrombin, atrial natriuretic peptide, vasopressin and serotonin. 3. The whole-cell voltage clamp of dissociated cells revealed a transient increase of K+ conductance underlying the ATP and histamine responses. The agonist-induced current showed no time-dependent change during voltage steps. The response was showed no time-dependent change during voltage steps. The response was prevented by adding 10 mM EGTA to the pipette solution. 4. In the cell-attached single channel recordings, ATP induced transient K+ channel activities having a slope conductance of 34 pS. In inside-out patches, similar K+ channels were activated by applying Ca2+ of more than 0.1 microM. 5. These findings are consisten...Continue Reading

Citations

Apr 6, 2002·Cardiovascular Pathology : the Official Journal of the Society for Cardiovascular Pathology·Adam D Durbin, Avrum I Gotlieb
Dec 21, 2014·Purinergic Signalling·Geoffrey Burnstock, Amir Pelleg
Jan 1, 1997·Annual Review of Physiology·B NiliusG Droogmans
Jun 13, 2002·Clinical and Experimental Pharmacology & Physiology·Harold A ColemanHelena C Parkington
Feb 10, 1999·The American Journal of Physiology·A GattusoB Tota
Oct 3, 2001·Physiological Reviews·B Nilius, G Droogmans

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