Hyperpolarized Water Enhances Two-Dimensional Proton NMR Correlations: A New Approach for Molecular Interactions

Journal of the American Chemical Society
Aude SadetPaul R Vasos

Abstract

Protein and peptide interactions are characterized in the liquid state by multidimensional NMR spectroscopy experiments, which can take hours to record. We show that starting from hyperpolarized HDO, two-dimensional (2D) proton correlation maps of a peptide, either free in solution or interacting with liposomes, can be acquired in less than 60 s. In standard 2D NMR spectroscopy without hyperpolarization, the acquisition time required for similar spectral correlations is on the order of hours. This hyperpolarized experiment enables the identification of amino acids featuring solvent-interacting hydrogens and provides fast spectroscopic analysis of peptide conformers. Sensitivity-enhanced 2D proton correlation spectroscopy is a useful and straightforward tool for biochemistry and structural biology, as it does not recur to nitrogen-15 or carbon-13 isotope enrichment.

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Citations

Mar 12, 2020·Chemical Communications : Chem Comm·Hironori KounoNobuhiro Yanai
Jun 5, 2020·Chemical Communications : Chem Comm·Koki NishimuraNobuhiro Yanai
Jan 3, 2020·The Journal of Chemical Physics·Hanming YangSilvia Cavagnero
Jan 18, 2020·Proceedings of the National Academy of Sciences of the United States of America·Mihajlo NovakovicLucio Frydman
Feb 27, 2021·Progress in Nuclear Magnetic Resonance Spectroscopy·Florin TeleanuPaul R Vasos

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