Hyperspectral darkfield microscopy of PEGylated gold nanoparticles targeting CD44-expressing cancer cells

Journal of Biophotonics
Sergiy PatskovskyMichel Meunier

Abstract

We present a new hyperspectral darkfield imaging system with a scanned broadband supercontinuum light source. We observed the specific attachment of the functionalized gold plasmonic nanoparticles (AuNPs) targeting CD44(+) human breast cancer cells by conventional and by proposed hyperspectral darkfield microscopy. This wide-field and low phototoxic hyperspectral imaging system has been successful for performing spectral three-dimensional (3D) localization and spectroscopic identification of CD44-targeted PEGylated AuNPs in fixed cell preparations. Such spatial and spectral information is essential for the improvement of nanoplasmonic-based imaging, disease detection and treatment in complex biological environment. Presented system capability for 3D NP tracking will also enable investigation of specific sub-cellular activity with the use of NPs as spectral sensors.

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Citations

Jul 22, 2015·Expert Opinion on Drug Delivery·Zhi FangJiang-Feng Wu
Jun 30, 2015·The British Journal of Radiology·S W BotchwayF J Currell
Dec 1, 2017·Nature Communications·Maozhong SunChuanlai Xu
Sep 5, 2018·Chembiochem : a European Journal of Chemical Biology·Alejandro Méndez-ArdoyJavier Montenegro
Aug 1, 2019·Journal of Biophotonics·Lu WangMichel Meunier
Jul 30, 2020·Pharmaceutics·Moon Sung KangDong-Wook Han
Jun 24, 2016·Nanoscale·Éric BergeronMichel Meunier
Feb 13, 2018·Nanoscale·Valeria Rodríguez-FajardoRomain Quidant
May 26, 2018·Materials·Mario D'Acunto
Dec 16, 2018·International Journal of Molecular Sciences·Toni NunesAnne Janin
Sep 20, 2015·Journal of Biomedical Optics·Sergiy Patskovsky, Michel Meunier
Dec 18, 2020·Nanotechnology, Science and Applications·Ralf P FriedrichChristoph Alexiou
Jul 25, 2021·Cancers·Paule Marcoux-ValiquetteMichel Meunier

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