Hyperspectral Imaging for Burn Depth Assessment in an Animal Model

Plastic and Reconstructive Surgery. Global Open
Michael S ChinJanice F Lalikos

Abstract

Differentiating between superficial and deep-dermal (DD) burns remains challenging. Superficial-dermal burns heal with conservative treatment; DD burns often require excision and skin grafting. Decision of surgical treatment is often delayed until burn depth is definitively identified. This study's aim is to assess the ability of hyperspectral imaging (HSI) to differentiate burn depth. Thermal injury of graded severity was generated on the dorsum of hairless mice with a heated brass rod. Perfusion and oxygenation parameters of injured skin were measured with HSI, a noninvasive method of diffuse reflectance spectroscopy, at 2 minutes, 1, 24, 48 and 72 hours after wounding. Burn depth was measured histologically in 12 mice from each burn group (n = 72) at 72 hours. Three levels of burn depth were verified histologically: intermediate-dermal (ID), DD, and full-thickness. At 24 hours post injury, total hemoglobin (tHb) increased by 67% and 16% in ID and DD burns, respectively. In contrast, tHb decreased to 36% of its original levels in full-thickness burns. Differences in deoxygenated and tHb among all groups were significant (P < 0.001) at 24 hours post injury. HSI was able to differentiate among 3 discrete levels of burn injury. ...Continue Reading

References

Jan 1, 1992·World Journal of Surgery·D HeimbachJ Marvin
May 1, 1986·The Journal of Surgical Research·M J KorudaJ L Rombeau
Mar 1, 1984·Plastic and Reconstructive Surgery·M ChvapilT A Chvapil
May 1, 1984·The Journal of Trauma·D M HeimbachB Perry
Dec 1, 1993·Burns : Journal of the International Society for Burn Injuries·Z B NiaziM J Black
Jul 1, 1995·The Journal of Burn Care & Rehabilitation·L AtilesC Baxter
Jun 28, 2000·Burns : Journal of the International Society for Burn Injuries·F SjöbergW Monafo
Aug 10, 2000·The Journal of Burn Care & Rehabilitation·S IranihaB M Achauer
Apr 20, 2001·Burns : Journal of the International Society for Burn Injuries·M G SowaH H Mantsch
May 12, 2001·Burns : Journal of the International Society for Burn Injuries·J M StillJ Z Holtz
Sep 23, 2003·The Journal of Burn Care & Rehabilitation·Colin L RiordanLillian B Nanney
Oct 15, 2003·Burns : Journal of the International Society for Burn Injuries·J C JengM H Jordan
Nov 26, 2003·Burns : Journal of the International Society for Burn Injuries·L-P KamolzM Frey
Jun 1, 2007·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·K M CrossJ S Fish
Jul 7, 2007·Burns : Journal of the International Society for Burn Injuries·D J McGillS B Watson
May 31, 2008·Burns : Journal of the International Society for Burn Injuries·Stan MonstreyPhillip Blondeel
Aug 12, 2008·Burns : Journal of the International Society for Burn Injuries·M A AltintasP M Vogt
Oct 25, 2008·Burns : Journal of the International Society for Burn Injuries·Margit KempfRoy M Kimble
Oct 28, 2008·Burns : Journal of the International Society for Burn Injuries·Henk HoeksemaStan Monstrey
Nov 10, 2009·Journal of Burn Care & Research : Official Publication of the American Burn Association·Amín D JaskilleJames C Jeng
Dec 28, 2010·Burns : Journal of the International Society for Burn Injuries·Meghann KaiserAnthony J Durkin
Nov 26, 2011·Burns : Journal of the International Society for Burn Injuries·S A PapeS Monstrey
Apr 3, 2012·Journal of Biomedical Optics·Michael S ChinThomas J Fitzgerald
Sep 18, 2012·Burns : Journal of the International Society for Burn Injuries·Carmen GainesMark Van Dyke
Oct 4, 2012·Burns : Journal of the International Society for Burn Injuries·Yoo Seok ParkCheon Jae Yoon
Oct 13, 2012·Journal of Plastic, Reconstructive & Aesthetic Surgery : JPRAS·M TobalemR Wettstein
Apr 2, 2013·Plastic and Reconstructive Surgery·Michael S ChinJanice F Lalikos
Jun 15, 2013·Journal of Biomedical Optics·John Quan NguyenAnthony J Durkin
Nov 21, 2014·Journal of Burn Care & Research : Official Publication of the American Burn Association·Nicholas J PrindezeJeffrey W Shupp

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Citations

Mar 23, 2017·Journal of Burn Care & Research : Official Publication of the American Burn Association·Adam J Singer, Steven T Boyce
May 28, 2019·Journal of Biomedical Optics·Rebecca RowlandAnthony J Durkin
Sep 29, 2019·Clinical Hemorheology and Microcirculation·Eberhard GrambowMalte Weinrich
Nov 22, 2018·Biomedical Optics Express·Sorin Viorel ParascaRoxana Savastru
Apr 1, 2020·Advances in Wound Care·Shuxin LiLiping Tang
Dec 6, 2020·Burns : Journal of the International Society for Burn Injuries·Jörg MarotzFrank Siemers
May 7, 2020·Medical Image Analysis·Neil T ClancyDanail Stoyanov
Apr 30, 2021·Journal of Burn Care & Research : Official Publication of the American Burn Association·Melissa McCarthyJanice Lalikos
May 21, 2021·Journal of Burn Care & Research : Official Publication of the American Burn Association·Dominik PromnyBert Reichert

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Methods Mentioned

BETA
biopsy
biopsies
amputation

Software Mentioned

Image J
MATLAB
Stata

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