Hyperspectral optical tomography of intrinsic signals in the rat cortex

Neurophotonics
Soren D KoneckyBruce J Tromberg

Abstract

We introduce a tomographic approach for three-dimensional imaging of evoked hemodynamic activity, using broadband illumination and diffuse optical tomography (DOT) image reconstruction. Changes in diffuse reflectance in the rat somatosensory cortex due to stimulation of a single whisker were imaged at a frame rate of 5 Hz using a hyperspectral image mapping spectrometer. In each frame, images in 38 wavelength bands from 484 to 652 nm were acquired simultaneously. For data analysis, we developed a hyperspectral DOT algorithm that used the Rytov approximation to quantify changes in tissue concentration of oxyhemoglobin ([Formula: see text]) and deoxyhemoglobin (ctHb) in three dimensions. Using this algorithm, the maximum changes in [Formula: see text] and ctHb were found to occur at [Formula: see text] and [Formula: see text] beneath the surface of the cortex, respectively. Rytov tomographic reconstructions revealed maximal spatially localized increases and decreases in [Formula: see text] and ctHb of [Formula: see text] and [Formula: see text], respectively, with these maximum changes occurring at [Formula: see text] poststimulus. The localized optical signals from the Rytov approximation were greater than those from modified Be...Continue Reading

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Citations

Jul 12, 2007·PLoS Biology·Takashi R SatoKarel Svoboda
Mar 17, 2017·Journal of Biomedical Optics·Mohammad TorabzadehBruce J Tromberg
Jun 22, 2019·Journal of Biomedical Optics·Sylvain GiouxDavid J Cuccia
May 6, 2020·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Melika HosseiniYama Akbari

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