Hyperthermia-Induced Controlled Local Anesthesia Administration Using Gelatin-Coated Iron-Gold Alloy Nanoparticles

Pharmaceutics
Chien-Kun TingRen-Jei Chung

Abstract

The lack of optimal methods employing nanoparticles to administer local anesthesia often results in posing severe risks such as non-biocompatibility, in vivo cytotoxicity, and drug overdose to patients. Here, we employed magnetic field-induced hyperthermia to achieve localized anesthesia. We synthesized iron-gold alloy nanoparticles (FeAu Nps), conjugated an anesthetic drug, Lidocaine, and coated the product with gelatin to increase the biocompatibility, resulting in a FeAu@Gelatin-Lidocaine nano-complex formation. The biocompatibility of this drug-nanoparticle conjugate was evaluated in vitro, and its ability to trigger local anesthesia was also evaluated in vivo. Upon exposure to high-frequency induction waves (HFIW), 7.2 ± 2.8 nm sized superparamagnetic nanoparticles generated heat, which dissociated the gelatin coating, thereby triggering Lidocaine release. MTT assay revealed that 82% of cells were viable at 5 mg/mL concentration of Lidocaine, indicating that no significant cytotoxicity was induced. In vivo experiments revealed that unless stimulated with HFIW, Lidocaine was not released from the FeAu@Gelatin-Lidocaine complex. In a proof-of-concept experiment, an intramuscular injection of FeAu@Gelatin-Lidocaine complex wa...Continue Reading

References

Jun 1, 1996·General Pharmacology·A R Rigon, R N Takahashi
Nov 3, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Ravi ShuklaMurali Sastry
Aug 28, 2007·International Journal of Nanomedicine·Norah O'FarrellBenjamin R Horrocks
Mar 20, 2010·Journal of Materials Science. Materials in Medicine·Jui-Yang Lai
May 3, 2011·Journal of Colloid and Interface Science·Changwen ZhaoXuesi Chen
May 19, 2011·International Journal of Nanomedicine·Amel HaniniJulie Gavard
May 21, 2008·Journal of Physics. Condensed Matter : an Institute of Physics Journal·Michael LévyFlorence Gazeau
Dec 10, 2014·Nanoscale Research Letters·Sneha KrishnamurthyShivendra V Sahi
May 9, 2015·Applied Microbiology and Biotechnology·Richa SinghBalu A Chopade
Jul 15, 2015·Journal of Materials Science. Materials in Medicine·Nidhi Parikh, Kinnari Parekh
Oct 30, 2016·Revista brasileira de anestesiologia·Ding-Kun KangHong-Li Wang
Mar 21, 2018·ACS Applied Materials & Interfaces·Udesh DhawanWen Liang Chen
Dec 31, 2019·Saudi Pharmaceutical Journal : SPJ : the Official Publication of the Saudi Pharmaceutical Society·Suchipha WannaphatchaiyongWiwat Pichayakorn
Jan 7, 2014·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Ji LiuEtienne Duguet
Jul 11, 2020·Scientific Reports·Lígia N M RibeiroEneida de Paula
Nov 9, 2015·ACS Biomaterials Science & Engineering·Ece AlpaslanThomas J Webster

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Methods Mentioned

BETA
dynamic
X-ray
Infrared Spectroscopy
dynamic light scattering

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