Hyperthermia potentiates oncolytic herpes viral killing of pancreatic cancer through a heat shock protein pathway

Surgery
D P EisenbergYuman Fong

Abstract

Oncolytic herpes simplex virus-1 (HSV-1) is designed to specifically infect, replicate in, and lyse cancer cells. This study investigates a novel therapeutic regimen, combining the effects of NV1066 (a recombinant HSV-1) and hyperthermia in the treatment of pancreatic cancer. NV1066 is an attenuated HSV-1 that replicates in cells resistant to apoptosis. Heat shock protein 72 (Hsp72) is a member of a family of proteins that is upregulated after hyperthermic insult, lending cellular protection by inhibiting apoptosis. In these experiments, we test the hypothesis that increased Hsp72 expression in response to hyperthermia enhances anti-apoptotic mechanisms, thereby increasing viral replication and tumor cell kill. Hs 700T pancreatic cancer cells were treated with hyperthermia alone (42 degrees C), NV1066 alone, and combination therapy. Cell survival and viral growth were measured. The effect of siRNA-directed Hsp72 knockdown was also measured. Combining hyperthermia and viral treatment produced a synergistic effect on cell kill. Viral growth increased greater than 6-fold in the presence of hyperthermia (P < .05). Hyperthermia alone showed minimal cytotoxic activity against Hs 700T cells, while NV1066 infection resulted in approxim...Continue Reading

References

Nov 1, 1987·Photochemistry and Photobiology·J MattielloL Vandenheede
Nov 1, 1980·Radiology·H I BicherT O'Brien
Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·R ChambersR J Whitley
Jan 1, 1993·American Journal of Surgery·R J Geer, M F Brennan
Apr 14, 1999·CA: a Cancer Journal for Clinicians·S H LandisP A Wingo
Jul 31, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·D A KoobyY Fong
Dec 22, 1999·Biochemical Pharmacology·M G Santoro
Jan 6, 2000·Annals of Surgical Oncology·P H Sugarbaker, D Chang
Jun 27, 2000·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·J J BennettY Fong
Aug 10, 2000·Neoplasia : an International Journal for Oncology Research·A ChahlaviS D Rabkin
Feb 24, 2001·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·M H Falk, R D Issels
Apr 18, 2001·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·P F McAuliffeY Fong
Jul 10, 2001·Journal of the American College of Surgeons·R J WongY Fong
Nov 17, 2001·The Journal of Biological Chemistry·Nicolas Tanguy Le Gac, Paul E Boehmer
Mar 23, 2002·Cancer Treatment Reviews·A J WitkampF A Zoetmulder
Aug 16, 2002·Annals of Oncology : Official Journal of the European Society for Medical Oncology·J van der Zee
May 15, 2003·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·James R Lepock
May 15, 2003·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·M W DewhirstP J Hoopes
Dec 6, 2003·The Oncologist·Lynn A Gloeckler RiesBrenda K Edwards
Apr 3, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Joseph J BennettYuman Fong
Sep 16, 2004·Journal of Cell Science·Xian-Chun ZengEvan Eisenberg
Jan 22, 2005·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Se-Heon KimYuman Fong
Nov 5, 2005·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·David P EisenbergYuman Fong
Jan 5, 2006·Journal of the National Cancer Institute·Manish AghiRobert L Martuza
Feb 10, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Prasad S AdusumilliYuman Fong
Apr 4, 2007·The American Journal of Gastroenterology·Yasser ShaibHashem El-Serag
Nov 2, 2007·Journal of the American College of Surgeons·Maura ReinblattYuman Fong
Mar 5, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Shu-Fu LinRichard J Wong
Sep 20, 2008·Annals of Oncology : Official Journal of the European Society for Medical Oncology·V Heinemann, S Boeck
Apr 9, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Xue Qing LunJ Andrea McCart
May 14, 2009·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·Satoko AdachiToshikazu Yoshikawa

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Citations

Sep 2, 2015·Nature Reviews. Drug Discovery·Howard L KaufmanAndrew Zloza
Aug 16, 2016·Interdisciplinary Sciences, Computational Life Sciences·Asita Elengoe, Salehhuddin Hamdan
Jan 13, 2012·Journal of Virology·Andrea M MurphyValery Z Grdzelishvili
Feb 20, 2019·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·Jingjing SongXiaoming Yang

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