PMID: 9555828Apr 29, 1998Paper

Hypertonic saline activates lipid-primed human neutrophils for enhanced elastase release

The Journal of Trauma
D A PartrickC C Silliman

Abstract

Ongoing clinical trials have revived interest in hypertonic saline (HTS) for postinjury resuscitation; these studies have documented serum Na+ concentrations > or = 170 mmol/L. Recent animal studies have shown that HTS enhances T-cell and monocyte function, but effects on the polymorphonuclear neutrophil (PMN) remain unclear. The postinjury lipid mediators platelet-activating factor (PAF) and leukotriene B4 (LTB4) have been implicated in PMN priming for cytotoxicity, which is believed to be important in the pathogenesis of multiple organ failure. We hypothesized that HTS would stimulate PMN superoxide (O2-) and elastase release from PAF- and LTB4-primed PMNs. Isolated PMNs from five donors were primed for 5 minutes with 200 nmol/L PAF or 1 micromol/L LTB4 in Kreb's-Ringer's phosphate with dextrose at a Na+ concentration of 140 mmol/L (normal serum Na+ concentration), pelleted, and resuspended in Kreb's-Ringer's phosphate with dextrose for 10 minutes at a Na+ concentration of 130 to 170 mmol/L. O2- generation was measured by superoxide dismutase-inhibitable reduction of cytochrome c and elastase release by cleavage of N-methoxysuccinyl-Ala-Ala-Pro-Val p-nitroanilide. HTS with Na+ concentration up to 170 mmol/L had no significant...Continue Reading

References

Jun 1, 1991·The British Journal of Surgery·C R WelbournH B Hechtman
Jun 1, 1988·The Journal of Trauma·D GrossM M Krausz
Dec 1, 1994·American Journal of Respiratory and Critical Care Medicine·M JochumH Fritz
Apr 1, 1994·The Journal of Infectious Diseases·M B HamptonC C Winterbourn
Apr 1, 1993·Journal of Applied Physiology·R RabinoviciG Feuerstein
Dec 19, 1995·Proceedings of the National Academy of Sciences of the United States of America·L Shapiro, C A Dinarello
Jun 1, 1996·The Journal of Clinical Investigation·H KuboC M Doerschuk

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Citations

Jun 19, 2007·Neurosurgical Review·R TyagiJ Jallo
Feb 9, 2002·The Journal of Trauma·Scott L FahrnerLori Shatney-Leach
Mar 15, 2006·The Journal of Trauma·Raj BhatiaIan Pallister
Jan 26, 2010·Annales Françaises D'anesthèsie Et De Rèanimation·N LibertG Mion
Jun 16, 2004·Acta Anaesthesiologica Scandinavica·J A Kølsen-Petersen
Mar 5, 2004·Acta Anaesthesiologica Scandinavica·J A Kølsen-PetersenE Tonnesen
Oct 7, 2015·Daru : Journal of Faculty of Pharmacy, Tehran University of Medical Sciences·Javad MotahariniaMojtaba Mojtahedzadeh
Oct 15, 2010·Blood·Beth H ShazChristopher D Hillyer
Mar 10, 2001·The Journal of Trauma·J A DoyleD B Hoyt
Nov 12, 2005·American Journal of Physiology. Cell Physiology·Yu ChenWolfgang G Junger
May 9, 2002·American Journal of Physiology. Cell Physiology·Tatjana OrlicWolfgang G Junger

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