Abstract
Hypertonic saline infusion dampens inflammatory responses and suppresses neutrophil-endothelial interaction by reducing adhesion molecule expression. This study tested the hypothesis that hypertonic saline attenuates tumor cell adhesion to the endothelium through a similar mechanism. Human colon cancer cells (LS174T) were transfected with green fluorescent protein and exposed to lipopolysaccharide, tumor necrosis factor-alpha, and interleukin-6 under hypertonic and isotonic conditions for 1 and 4 hours. Confluent human umbilical vein endothelial cells were similarly exposed. Cellular apoptosis and expression of adhesion molecules and laminin were measured by flow cytometry. Tumor cell adhesion to endothelium and laminin was assessed with fluorescence microscopy. Data are represented as mean +/- standard error of mean, and an ANOVA test was performed to gauge statistical significance, with P <.05 considered significant. Hypertonic exposure significantly reduced tumor cell adhesion despite the presence of the perioperative cell stressors (42 +/- 2.9 vs 172.5 +/- 12.4, P <.05), attenuated tumor cell beta-1 integrin (14.43 vs 23.84, P <.05), and endothelial cell laminin expression (22.78 +/- 2.2 vs 33.74 +/- 2.4, P <.05), but did n...Continue Reading
References
Apr 3, 1992·Cell·R O Hynes
Jul 1, 1989·The Journal of Cell Biology·R O HynesG H Yee
May 1, 1995·Clinical & Experimental Metastasis·R D BlissT W Lennard
Jan 1, 1993·Journal of Cardiovascular Pharmacology·E Dejana
Feb 7, 1998·Hypertension·Y KimA J Lonigro
Apr 3, 1998·Shock·N AngleW G Junger
Jun 24, 1998·The Journal of Clinical Investigation·W G JungerA Altman
Nov 17, 1998·Medicina·M Rocha e Silva
Dec 16, 1998·Neurosurgery·T J MacDonaldW E Laug
May 18, 1999·The Journal of Surgical Research·S B RizoliO D Rotstein
Jul 27, 1999·The Journal of Biological Chemistry·S B RizoliA Kapus
Aug 24, 1999·Surgery·S SookhaiH P Redmond
Dec 22, 1999·British Journal of Cancer·G P PidgeonD J Bouchier-Hayes
Apr 1, 2000·The Journal of Trauma·D J CieslaC C Silliman
Jul 26, 2000·Shock·Y MuraoW G Junger
Oct 24, 2000·The British Journal of Surgery·C J ShieldsH P Redmond
Apr 26, 2001·The Journal of Surgical Research·E J AndrewsH P Redmond
Apr 27, 2001·Seminars in Cancer Biology·J KassisA Wells
Sep 27, 2001·Journal of Cellular Physiology·E H Danen, K M Yamada
Jan 10, 2002·Biochimica Et Biophysica Acta·U Cavallaro, G Christofori
Oct 18, 2002·The Journal of Surgical Research·J F DowdallH P Redmond
Jan 7, 2003·Archives of Surgery·Conor J ShieldsH Paul Redmond
Feb 21, 2003·Surgical Infections·O D Rotstein
Feb 21, 2003·Surgical Infections·C J ShieldsH P Redmond
Citations
Sep 22, 2006·The Journal of Membrane Biology·Conor J ShieldsH Paul Redmond
Jun 21, 2005·Journal of Biomedical Materials Research. Part a·Min-Huey ChenTai-Horng Young
Mar 23, 2005·European Journal of Surgical Oncology : the Journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·A M HanlyD J Bouchier-Hayes
Mar 3, 2009·Tissue Engineering. Part C, Methods·Joo Youn OhJin Hak Lee
Apr 6, 2006·Pflügers Archiv : European journal of physiology·Stine Falsig Pedersen
Jan 8, 2009·Physiological Reviews·Else K HoffmannStine F Pedersen
Jul 18, 2020·Scientific Reports·Hande KaramahmutogluMeltem Elitas