Hypertonic saline impedes tumor cell-endothelial cell interaction by reducing adhesion molecule and laminin expression

Surgery
Conor J ShieldsH Paul Redmond

Abstract

Hypertonic saline infusion dampens inflammatory responses and suppresses neutrophil-endothelial interaction by reducing adhesion molecule expression. This study tested the hypothesis that hypertonic saline attenuates tumor cell adhesion to the endothelium through a similar mechanism. Human colon cancer cells (LS174T) were transfected with green fluorescent protein and exposed to lipopolysaccharide, tumor necrosis factor-alpha, and interleukin-6 under hypertonic and isotonic conditions for 1 and 4 hours. Confluent human umbilical vein endothelial cells were similarly exposed. Cellular apoptosis and expression of adhesion molecules and laminin were measured by flow cytometry. Tumor cell adhesion to endothelium and laminin was assessed with fluorescence microscopy. Data are represented as mean +/- standard error of mean, and an ANOVA test was performed to gauge statistical significance, with P <.05 considered significant. Hypertonic exposure significantly reduced tumor cell adhesion despite the presence of the perioperative cell stressors (42 +/- 2.9 vs 172.5 +/- 12.4, P <.05), attenuated tumor cell beta-1 integrin (14.43 vs 23.84, P <.05), and endothelial cell laminin expression (22.78 +/- 2.2 vs 33.74 +/- 2.4, P <.05), but did n...Continue Reading

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Citations

Jun 21, 2005·Journal of Biomedical Materials Research. Part a·Min-Huey ChenTai-Horng Young
Mar 23, 2005·European Journal of Surgical Oncology : the Journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·A M HanlyD J Bouchier-Hayes
Mar 3, 2009·Tissue Engineering. Part C, Methods·Joo Youn OhJin Hak Lee
Apr 6, 2006·Pflügers Archiv : European journal of physiology·Stine Falsig Pedersen
Jan 8, 2009·Physiological Reviews·Else K HoffmannStine F Pedersen

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