PMID: 7545304Aug 29, 1995Paper

Hypertonicity activates nonselective cation channels in mouse cortical collecting duct cells

Proceedings of the National Academy of Sciences of the United States of America
T VolkC Korbmacher

Abstract

We investigated the effect of cell shrinkage on whole-cell currents of M-1 mouse cortical collecting duct cells. Addition of 100 mM sucrose to an isotonic NaCl bath solution induced cell shrinkage and increased whole-cell currents within 5-10 min by approximately 12-fold. The effect was reversible upon return to isotonic solution and could also be elicited by adding 100 mM urea or 50 mM NaCl. Replacement of bath Na+ by K+, Cs+, Li+, or Rb+ did not significantly affect the stimulated inward current, but replacement by N-methyl-D-glucamine reduced it by 88.1 +/- 1.3% (n = 34); this demonstrates that hypertonicity activates a nonselective alkali cation conductance. The activation was independent of extra- and intracellular Ca2+, but 1 or 10 mM ATP in the pipette suppressed it in a concentration-dependent manner, indicating that intracellular ATP levels may modulate the degree of channel activation. Flufenamic acid (0.1 mM) and gadolinium (0.1 mM) inhibited the stimulated current by 68.7 +/- 5.9% (n = 9) and 32.4 +/- 11.7% (n = 6), respectively, whereas 0.1 mM amiloride had no significant effect. During the early phase of hypertonic stimulation single-channel transitions could be detected in whole-cell current recordings, and a gra...Continue Reading

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Citations

Oct 26, 2005·Naunyn-Schmiedeberg's Archives of Pharmacology·Philipp A LangChristophe Duranton
Dec 20, 2005·The Journal of Membrane Biology·F LangS M Huber
Jun 1, 2006·Acta Physiologica·F WehnerT Li
Jan 11, 2012·Biochimica Et Biophysica Acta·Romain GuinamardJacques Teulon
Apr 18, 2003·Cell Death and Differentiation·K S LangS M Huber
Mar 5, 2005·Cell Death and Differentiation·P A LangS M Huber
Jan 8, 2009·Physiological Reviews·Else K HoffmannStine F Pedersen
Feb 3, 2005·The Journal of Pharmacology and Experimental Therapeutics·Yukun Yuan, William D Atchison
Mar 20, 2010·American Journal of Physiology. Cell Physiology·Sergios GatidisFlorian Lang
Jul 7, 2017·Clinical and Experimental Pharmacology & Physiology·Xiao-Cao LiuMeng-Fei Yu
May 8, 2004·American Journal of Physiology. Renal Physiology·Karl S LangChristophe Duranton
Feb 11, 1998·Physiological Reviews·F LangD Häussinger
Dec 22, 1999·The American Journal of Physiology·R M RomanJ G Fitz
Mar 29, 2001·Physiological Reviews·O P Hamill, B Martinac
Apr 24, 2003·American Journal of Physiology. Renal Physiology·Jean-Yves LapointeYasunobu Okada
Sep 8, 2001·American Journal of Physiology. Cell Physiology·C A WagnerS Bröer
Mar 4, 2020·The Journal of Biological Chemistry·Alexandria O MiltonShetuan Zhang
Oct 22, 2003·Journal of the American Society of Nephrology : JASN·Svetlana MyssinaFlorian Lang
Feb 24, 2007·Archives of Biochemistry and Biophysics·Florian LangErich Gulbins

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