PMID: 9436570Jan 22, 1998Paper

Hypertonicity induces injury to cultured human endothelium: attenuation by glutamine

The Annals of Thoracic Surgery
A ParolariP Biglioli

Abstract

Although most preservation solutions as well as some cardioplegic solutions used for organ storage and transplantation are hypertonic, the effects of extracellular hypertonicity on endothelium are not well established. Aims of this study were to evaluate the response of cultured human saphenous vein endothelial cells to extracellular hypertonicity and to investigate the role of the amino acid glutamine in preventing endothelial damage in vitro. Eight distinct strains of human saphenous vein endothelial cells were studied. Hypertonic (350 and 400 mosm/kg) media were obtained by supplementing culture medium with sucrose. Cell viability was assessed in the absence or the presence of glutamine through the determination of cell number and protein content of the cultures. Confocal microscopy of cells loaded with the fluorescent dye calcein was also performed. Exposure of human saphenous vein endothelial cells to hypertonic media without glutamine caused significant cell loss within 30 minutes. Cell loss progressed steadily during incubation and after 6 hours reached 50% at 350 mosm/kg and 65% at 400 mosm/kg. In the presence of 2 mmol/L glutamine, endothelial damage was completely prevented at 350 mosm/kg and significantly lessened at...Continue Reading

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Citations

May 23, 2002·The Annals of Thoracic Surgery·Francesco AlamanniValeria Dall'Asta
Jul 9, 2004·Biochimica Et Biophysica Acta·Rossana VisigalliValeria Dall'Asta
Sep 26, 2006·The Annals of Thoracic Surgery·Kazuhiro HinokiyamaJarle Vaage
Dec 3, 2014·The Journal of Surgical Research·Mihai OlteanAnna Casselbrant
Apr 3, 2007·Biochimica Et Biophysica Acta·Rossana VisigalliValeria Dall'Asta
Dec 20, 2000·The Journal of Surgical Research·K S BiswasS F Khuri
Aug 24, 1999·Current Opinion in Clinical Nutrition and Metabolic Care·R D Griffiths
Dec 18, 2001·American Journal of Physiology. Cell Physiology·Roberto SalaValeria Dall'Asta
Apr 1, 2003·Endocrine Research·G F CarlMary H Jonah
Oct 30, 2001·The Proceedings of the Nutrition Society·R D Griffiths

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