Hypertrophic stimulation increases beta-actin dynamics in adult feline cardiomyocytes.

PloS One
Sundaravadivel BalasubramanianD Kuppuswamy

Abstract

The myocardium responds to hemodynamic stress through cellular growth and organ hypertrophy. The impact of cytoskeletal elements on this process, however, is not fully understood. While alpha-actin in cardiomyocytes governs muscle contraction in combination with the myosin motor, the exact role of beta-actin has not been established. We hypothesized that in adult cardiomyocytes, as in non-myocytes, beta-actin can facilitate cytoskeletal rearrangement within cytoskeletal structures such as Z-discs. Using a feline right ventricular pressure overload (RVPO) model, we measured the level and distribution of beta-actin in normal and pressure overloaded myocardium. Resulting data demonstrated enriched levels of beta-actin and enhanced translocation to the Triton-insoluble cytoskeletal and membrane skeletal complexes. In addition, RVPO in vivo and in vitro hypertrophic stimulation with endothelin (ET) or insulin in isolated adult cardiomyocytes enhanced the content of polymerized fraction (F-actin) of beta-actin. To determine the localization and dynamics of beta-actin, we adenovirally expressed GFP-tagged beta-actin in isolated adult cardiomyocytes. The ectopically expressed beta-actin-GFP localized to the Z-discs, costameres, and cel...Continue Reading

References

Jan 1, 1990·Clinical and Experimental Hypertension. Part A, Theory and Practice·M I CherninK M Baker
Nov 1, 1974·Journal of Applied Physiology·G Cooper, R M Satava
Apr 1, 1981·Circulation Research·G CooperM L Marcus
Jan 1, 1994·Cell Motility and the Cytoskeleton·D RheeJ W Sanger
Feb 14, 1997·The Journal of Biological Chemistry·D KuppuswamyG Cooper
Feb 1, 1997·Cell Structure and Function·Y ShimadaA Konno
Feb 7, 1998·Hypertension·W A HsuehY S Do
Nov 26, 1998·Cell Motility and the Cytoskeleton·B A RenleyJ M Ervasti
Aug 26, 2000·The Journal of Biological Chemistry·M LaserD Kuppuswamy
Nov 4, 2000·International Review of Cytology·S Y Khaitlina
Feb 22, 2001·Annual Review of Physiology·J D Molkentin, G W Dorn
Jun 5, 2001·Nature Cell Biology·R LittlefieldV M Fowler
Jun 29, 2002·Circulation Research·Dhandapani Kuppuswamy
Aug 6, 2002·Molecular Therapy : the Journal of the American Society of Gene Therapy·Yasufumi KanedaHitoshi Kotani
Jun 6, 2003·Journal of Molecular and Cellular Cardiology·Christopher D WilleyDhandapani Kuppuswamy
Aug 12, 2003·The Journal of Biological Chemistry·Sundaravadivel Balasubramanian, Dhandapani Kuppuswamy
Sep 27, 2003·Nucleic Acids Research. Supplement·Javier MartinezThomas Tuschl
Jun 8, 2004·Current Cancer Drug Targets·JianYu Rao, Ning Li
Oct 7, 2004·Nature Cell Biology·Estela JacintoMichael N Hall
Nov 3, 2004·Nature Reviews. Genetics·Choong-Chin Liew, Victor J Dzau
Apr 6, 2005·Cell Motility and the Cytoskeleton·Jushuo WangJoseph W Sanger
Jul 5, 2005·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Ji-Guo Yu, Brenda Russell
Nov 15, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Allen M Samarel
Feb 10, 2006·Cardiovascular Research·Mara BrancaccioGuido Tarone
Apr 1, 2006·Circulation Research·Joan Heller BrownMark A Sussman
May 3, 2006·Proceedings of the National Academy of Sciences of the United States of America·Minoru SatohJames K Liao
Aug 22, 2006·Biophysical Journal·Yunfei CaiMichael P Sheetz
Aug 25, 2006·Journal of Molecular and Cellular Cardiology·Sundaravadivel BalasubramanianDhandapani Kuppuswamy
Dec 23, 2006·Journal of Molecular and Cellular Cardiology·Jie RenAnthony J Muslin
May 4, 2007·Neuromuscular Disorders : NMD·Laurin M HanftJames M Ervasti
Jul 28, 2007·Tsitologiia·S Iu Khaĭtlina
Oct 24, 2007·Basic Research in Cardiology·William J TuxworthDhandapani Kuppuswamy
Nov 6, 2007·Molecular Biology of the Cell·Olivier DestaingRoland Baron
Sep 17, 2008·Seminars in Cell & Developmental Biology·Ryan S Littlefield, Velia M Fowler
Jan 2, 2009·Journal of Cell Science·Miguel Vicente-ManzanaresAlan Rick Horwitz
Jan 20, 2009·Cardiovascular & Hematological Agents in Medicinal Chemistry·Sundaravadivel BalasubramanianDhandapani Kuppuswamy

❮ Previous
Next ❯

Citations

Jun 2, 2012·Frontiers in Bioscience (Landmark Edition)·Qinchuan WangChung-Jing J Lin
Jun 23, 2015·Journal of Cellular Physiology·Valeria De ArcangelisLucia Monaco
Apr 16, 2015·Evidence-based Complementary and Alternative Medicine : ECAM·Sundaravadivel BalasubramanianGraham W Warren
Mar 14, 2018·American Journal of Physiology. Endocrinology and Metabolism·Agnete B MadsenThomas E Jensen
Sep 18, 2013·American Journal of Physiology. Heart and Circulatory Physiology·Jieli Li, Brenda Russell
Jul 29, 2011·The Journal of Biological Chemistry·Sudha TalwarViswanathan Palanisamy

❮ Previous
Next ❯

Methods Mentioned

BETA
fluorescent
GTPases
confocal microscopy
fluorescence recovery after photobleaching
atomic force microscopy
GTPase

Software Mentioned

IonWizard
IonOptix
SoftEdge
Kaleidagraph

Related Concepts

Related Feeds

Cardiomegaly

Cardiomegaly, known as an enlarged heart, is a multifactorial disease with different pathophysiological mechanisms. Hypertension, pregnancy, exercise-induced and idiopathic causes are some mechanisms of cardiomegaly. Discover the latest research of cardiomegaly here.

Actin-binding Proteins

Actin-binding proteins are a component of the actin cytoskeleton that play essential roles in cellular functions such as regulation of actin polymerization, maintenance of cell polarity, gene expression regulation, cell motility and many more functions. Discover the latest research on actin-binding proteins here.

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.

Cardiomyopathy

Cardiomyopathy is a disease of the heart muscle, that can lead to muscular or electrical dysfunction of the heart. It is often an irreversible disease that is associated with a poor prognosis. There are different causes and classifications of cardiomyopathies. Here are the latest discoveries pertaining to this disease.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.