Hypertrophy and transcriptional regulation induced in myogenic cell line L6-C5 by an increase of extracellular calcium

Journal of Cellular Physiology
Vania De ArcangelisFabio Naro

Abstract

Calcium plays a pivotal role in the establishment of the differentiated phenotype in myogenic cells but the involved molecular mechanisms are still matter of debate. Here we studied the effects of exposing L6-C5 myogenic cells to high extracellular Ca2+ concentration ([Ca2+]o), which induces an increase of intracellular calcium ([Ca2+]i) without involving Ca2+ release from the intracellular stores but exclusively due to plasma membrane influx (Naro et al., 2003). Exposure of L6-C5 cells to [Ca2+]o up to 20 mM for 30 min, before shifting them into a differentiative medium, induced the appearance of multinucleated, myosin-positive myotubes, much larger than in control cells with an increased protein/DNA ratio. These large myotubes showed nuclear accumulation of the hypertrophy marker GATA-2. The hypertrophic growth of these cells was blocked by cyclosporin A (CsA), FK506, or overexpression of a calcineurin-dominant negative protein, suggesting the involvement in this process of the Ca2+ responsive phosphatase calcineurin. Furthermore, transient exposure of L6-C5 cells to high [Ca2+]o increased the expression of luciferase reporter driven by myoglobin (Mb) and beta-MHC promoters but not IIB-MHC and MCK promoters. Luciferase transc...Continue Reading

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Aug 3, 2010·The Journal of Experimental Biology·Shane B Kanatous, Pradeep P A Mammen
Nov 13, 2012·PloS One·Valentina BizzarroAntonello Petrella
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Jan 8, 2009·Medicine and Science in Sports and Exercise·Timothy A Butterfield, Thomas M Best
Feb 14, 2007·Journal of Cellular Physiology·Lucia FormigliSandra Zecchi-Orlandini

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