Hyphal growth in Candida albicans requires the phosphorylation of Sec2 by the Cdc28-Ccn1/Hgc1 kinase.

The EMBO Journal
Amy BishopPeter Sudbery

Abstract

Polarized growth is a fundamental property of cell growth and development. It requires the delivery of post-Golgi secretory vesicles to the site of polarized growth. This process is mediated by Rab GTPases activated by their guanine exchange factors (GEFs). The human fungal pathogen, Candida albicans, can grow in a budded yeast form or in a highly polarized hyphal form, and thus provides a model to study this phenomenon. During hyphal, but not yeast growth, secretory vesicles accumulate in an apical body called a Spitzenkörper, which acts to focus delivery of the vesicles to the tip. Post-Golgi transport of secretory vesicles is mediated by the Rab GTPase Sec4, activated by its GEF Sec2. Using a combination of deletion mapping, in vitro mutagenesis, an analogue-sensitive allele of Cdc28 and an in vitro kinase assay, we show that localization of Sec2 to the Spitzenkörper and normal hyphal development requires phosphorylation of Serine 584 by the cyclin-dependent kinase Cdc28. Thus, as well as controlling passage through the cell cycle, Cdc28 has an important function in controlling polarized secretion.

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Citations

May 4, 2011·The Journal of Microbiology·Joon Kim, Peter Sudbery
Mar 19, 2013·Journal of Inorganic Biochemistry·Brandon J HeilmanPradip K Mascharak
Jul 23, 2011·Nature Reviews. Molecular Cell Biology·Harvey T McMahon, Emmanuel Boucrot
Dec 14, 2011·Nature Reviews. Microbiology·Neil A R GowMihai G Netea
Jun 8, 2011·Microbiology and Molecular Biology Reviews : MMBR·Rebecca S ShapiroLeah E Cowen
Jan 26, 2013·Scientifica·James B Konopka
Feb 7, 2014·Molecular Biology of the Cell·David Caballero-Lima, Peter E Sudbery
Sep 30, 2014·Trends in Microbiology·Yang LuHaoping Liu
Nov 20, 2013·Proceedings of the National Academy of Sciences of the United States of America·Danièle StalderPeter J Novick
Aug 15, 2012·The Journal of Cell Biology·Aurélia VernayRobert A Arkowitz
Dec 24, 2014·Proceedings of the National Academy of Sciences of the United States of America·Juanfei WangWei Guo
Sep 13, 2011·Seminars in Cell & Developmental Biology·Robert A Arkowitz, Martine Bassilana
Mar 3, 2011·Fungal Genetics and Biology : FG & B·Peter Sudbery
Jul 24, 2015·Scientific Reports·Chaminda Jayampath SeneviratneYue Wang
Sep 19, 2014·Molecular Microbiology·David Caballero-LimaPeter E Sudbery
Jun 21, 2015·Fungal Genetics and Biology : FG & B·M GuoG Steinberg
Jan 23, 2015·Molecular Biology of the Cell·Shamoon NaseemJames B Konopka
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Jan 28, 2017·Small GTPases·Neus PedrazaFrancisco Ferrezuelo
Jan 27, 2018·Journal of Fungi·Jigar V Desai
Aug 17, 2011·Nature Reviews. Microbiology·Peter E Sudbery
Dec 28, 2019·Journal of Fungi·Kyunghun MinJames B Konopka
Feb 28, 2020·Journal of Fungi·Christiane RollenhagenSamuel A Lee
Aug 24, 2019·Frontiers in Microbiology·Floris Schoeters, Patrick Van Dijck
May 5, 2020·Journal of Extracellular Vesicles·Charlotte S DawsonMarilyn A Anderson
Aug 15, 2020·Scientific Reports·Ronit Vogt SionovDoron Steinberg
Feb 28, 2016·The Journal of Microbiology·Lois M Douglas, James B Konopka

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