Hyphenated LC-MALDI-ToF/ToF and LC-ESI-QToF approach in proteomic characterization of honeybee venom

Journal of Pharmaceutical and Biomedical Analysis
Jan MatysiakZenon J Kokot

Abstract

To increase in the depth characterization of venom proteome of Apis mellifera the hyphenated LC-MALDI-ToF/ToF-MS (liquid chromatography-matrix-assisted laser desorption/ionization-time of flight/time of flight tandem mass spectrometry) and LC-ESI-QToF-MS (liquid chromatography-electrospray ionization-quadrupole time of flight tandem mass spectrometry) techniques combined with combinatorial peptide ligand library enrichment method is proposed in this study. The novel approach simplifies pretreatment protocol in venom investigation. By using the protein preparation kit with sequential multi-step elution, the honeybee venom was dispensed into four different fractions. In total 269 proteins were detected, among these 49 honeybee toxins, allergens and components involved in mechanism of envenoming belonging to venom enzyme classes of esterases, proteases/peptidases, protease inhibitors, hydrolases and major royal jelly proteins. Moreover 5 additional putative toxins were identified. Their role in envenoming process was discussed. We concluded that different mass spectrometry techniques increased the detection of the honeybee venom proteins, underscoring the complementary character of analytical methods. The combination of MALDI and ...Continue Reading

References

Oct 29, 1998·Cellular and Molecular Life Sciences : CMLS·J SchmitzováJ Simúth
Aug 23, 2005·Biochimica Et Biophysica Acta·Nico PeirenFrans J Jacobs
Apr 1, 2006·Toxicon : Official Journal of the International Society on Toxinology·Jay W FoxSolange M T Serrano
May 24, 2006·Journal of Mass Spectrometry : JMS·Armin HolleJens Höhndorf
Sep 23, 2006·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Seok-Jong SuhCheorl-Ho Kim
Jul 11, 2007·Journal of Mass Spectrometry : JMS·Juan J CalveteLibia Sanz
Oct 24, 2007·Nature Methods·Lukas KällMichael J MacCoss
Jun 25, 2008·Toxicon : Official Journal of the International Society on Toxinology·Nico PeirenFrans J Jacobs
Jul 16, 2008·Journal of Proteomics·Xiangming Fang, Wei-Wei Zhang
Oct 14, 2008·Journal of the American Society for Mass Spectrometry·Simona FranceseStefano Turillazzi
Dec 30, 2008·Journal of Chromatography. a·Egisto BoschettiPier Giorgio Righetti
Apr 7, 2009·Journal of Proteomics·Dirk C de GraafBart Devreese
Apr 24, 2009·Journal of Proteome Research·Marina SpivakWilliam Stafford Noble
Sep 21, 2010·Journal of Pharmaceutical and Biomedical Analysis·Jan MatysiakZenon J Kokot
Dec 8, 2010·Proteomics. Clinical Applications·Marie FertinFlorence Pinet
Jan 12, 2011·Analytical and Bioanalytical Chemistry·Zenon J KokotPaweł Dereziński
Mar 24, 2012·The Journal of Experimental Biology·Heike GätschenbergerHildburg Beier
Jul 21, 2012·Journal of Proteomics·Youcef ShahaliPascal Poncet
Jun 27, 2013·Proteomics·Virgínia Maria Ferreira ResendeAndrej Shevchenko
Dec 10, 2013·Journal of Proteomics·Pier Giorgio Righetti
Aug 16, 2014·Journal of Proteomics·Laura SantucciGian Marco Ghiggeri
Sep 1, 2014·Toxicon : Official Journal of the International Society on Toxinology·Jan MatysiakZenon J Kokot
Jun 14, 2015·Toxicon : Official Journal of the International Society on Toxinology·Matthias Van VaerenberghDirk C de Graaf

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Citations

Sep 27, 2018·Bioscience Reports·Lin ChaiZhicheng Fang
Sep 5, 2019·International Journal of Molecular Sciences·Solomon Zewdu AltayeJianke Li
Jun 23, 2019·Scientific Reports·Carmen I Mureşan, Anja Buttstedt
Dec 12, 2020·Scientific Reports·Agnieszka KlupczynskaJan Matysiak
Apr 11, 2021·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Bo Yeon KimByung Rae Jin
Jun 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Eliza MatuszewskaJan Matysiak

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