Hyphenation of capillary high-performance liquid chromatography with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for nano-scale screening of single-bead combinatorial libraries

Rapid Communications in Mass Spectrometry : RCM
Oliver KeilDietrich A Volmer

Abstract

This paper focuses on the technical aspects of chemical screening from 384-well plate nano-scale single-bead combinatorial libraries. The analytical technique utilized is a combination of capillary liquid chromatography with ultraviolet detection and matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. The HPLC/MALDI-MS hyphenation is achieved by means of a micro-fraction collector with a peak detection system that automatically collects the peaks onto the MALDI targets for subsequent characterization. Several experimental parameters such as type of 384-well plate, well-plate sealing foils, and a column-switching procedure were investigated using a small test library of nine components. Additionally, the influence of different MALDI matrices, different MALDI targets and sample-spotting techniques on the MALDI detection sensitivity as well as the ruggedness and sample throughput capacity of this technique were studied. Optimum results for the analytes investigated were obtained with 2,5-dihydroxybenzoic acid using on-line mixing of HPLC effluent and matrix solution. To demonstrate the potential of this capillary HPLC/MALDI-TOFMS method, its application to several single-bead libraries was investigated. The inst...Continue Reading

References

Jul 21, 2000·Mass Spectrometry Reviews·C EnjalbalJ L Aubagnac

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Citations

Oct 15, 2003·Rapid Communications in Mass Spectrometry : RCM·Panos HatsisDietrich A Volmer
Sep 19, 2007·Journal of Separation Science·Ivan TalianHeinrich F Arlinghaus
Apr 18, 2013·Rapid Communications in Mass Spectrometry : RCM·David Gode, Dietrich A Volmer
Aug 4, 2004·Current Opinion in Chemical Biology·Jeremy R Kenseth, Shelley J Coldiron
Jul 19, 2002·Journal of Mass Spectrometry : JMS
Jul 15, 2003·Journal of Combinatorial Chemistry·Michael A R MeierUlrich S Schubert

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