Hypocalcemia increases and hypercalcemia decreases the steady-state level of parathyroid hormone messenger RNA in the rat

The Journal of Clinical Investigation
M YamamotoE Ogata

Abstract

To examine the effects of serum calcium concentrations on PTH biosynthesis, rats were made hyper- (serum total calcium, approximately 3.5 mM) or hypocalcemic (approximately 1.25 mM) and steady-state levels of PTH mRNA in parathyroid cells were measured by the primer extension method using a 32P-labeled synthetic oligomer. PTH mRNA levels increased about twofold in the rats made slightly hypocalcemic by infusion of calcium-free solution and decreased slightly in those made hypercalcemic by CaCl2 infusion (120-150 mumol/h) compared with the levels present in nonfasting control rats. Infusion of calcitonin (0.5 U/h) or EGTA (90 mumol/h) with calcium-free solution increased PTH mRNA levels further (two- to sevenfold) above the levels present in animals infused with calcium-free solution alone. These changes in PTH mRNA levels were observed after 48- but not 24-h infusion, and there was an inverse correlation between PTH mRNA levels and serum calcium concentrations. The results suggest that changes in serum calcium concentrations in the near physiological range regulate the biosynthesis of PTH by affecting steady-state levels of PTH mRNA when hypercalcemia or hypocalcemia continues for a relatively long period.

References

Mar 11, 1976·The Journal of Clinical Endocrinology and Metabolism·J F HabenerJ T Potts
Dec 1, 1986·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J J BrookmanG N Hendy
Nov 1, 1986·The Journal of Clinical Investigation·J SilverM M Popovtzer
Jun 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J SilverL M Sherwood
Aug 1, 1984·The Journal of Clinical Investigation·M YamamotoE Ogata
Mar 25, 1983·Nucleic Acids Research·U NudelD Yaffe

❮ Previous
Next ❯

Citations

Sep 1, 1992·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M CloutierP D'Amour
Oct 1, 1992·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·T Naveh-ManyJ Silver
Mar 1, 1995·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·E MoallemT Naveh-Many
Jul 1, 1996·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Y AlmadenM Rodriguez
Sep 1, 1996·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·E Aguilera-TejeroA J Felsenfeld
Jan 1, 1991·Calcified Tissue International·M LadizeskyC A Mautalen
Mar 28, 2008·International Urology and Nephrology·Csaba P Kovesdy, Kamyar Kalantar-Zadeh
Jan 1, 1996·Archives of Gerontology and Geriatrics·G BonannoP Fatuzzo
Feb 28, 2003·General and Comparative Endocrinology·Ramiro E ToribioThomas J Rosol
Jan 14, 2000·Kidney International. Supplement·J SilverT Naveh-Many
Apr 10, 2008·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Andreas PaschOlivier Bonny
Jun 17, 2008·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Natalia Carrillo-LópezJorge B Cannata-Andía
Aug 10, 2001·Current Opinion in Nephrology and Hypertension·W G Goodman
Dec 1, 2005·Kidney International·Ignacio González-SuárezJorge B Cannata-Andía
Aug 10, 2007·Molekuliarnaia biologiia·N S DyrkheevaO I Lavrik
Oct 1, 1990·The Journal of Clinical Investigation·T Naveh-Many, J Silver
Jul 1, 1995·The Journal of Clinical Investigation·R KilavT Naveh-Many
Nov 26, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J C EstepaA J Felsenfeld
Feb 24, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·D HunterT D Spector
Dec 10, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Grahame Elder
Mar 8, 2006·Clinical Chemistry and Laboratory Medicine : CCLM·Indra Ramasamy
Jul 24, 2010·Journal of the American Society of Nephrology : JASN·Loan Nguyen-YamamotoDavid Goltzman
Dec 21, 2013·Molecular Biology of the Cell·Giulia AntonialiGianluca Tell
Aug 14, 2010·Cellular and Molecular Life Sciences : CMLS·Gianluca TellFranco Quadrifoglio
Nov 11, 1991·World Journal of Surgery·M BackdahlS A Wells
Feb 22, 2008·Kidney International·C P Kovesdy, K Kalantar-Zadeh
Apr 22, 2009·Therapeutic Apheresis and Dialysis : Official Peer-reviewed Journal of the International Society for Apheresis, the Japanese Society for Apheresis, the Japanese Society for Dialysis Therapy·Michio KuwaharaSei Sasaki
Sep 5, 2008·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Karin WeberReinhold G Erben
Jul 30, 2011·Bone·Ayfer Gözü PirinççioğluMurat Söker
Aug 4, 2006·European Journal of Clinical Investigation·A Ferreira
Aug 19, 2007·Clinical Journal of the American Society of Nephrology : CJASN·Csaba P KovesdyKamyar Kalantar-Zadeh

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.