Hypochlorite-modified low-density lipoprotein stimulates human polymorphonuclear leukocytes for enhanced production of reactive oxygen metabolites, enzyme secretion, and adhesion to endothelial cells

Atherosclerosis
Steffi KoppraschH Kühne

Abstract

Hypochlorite-oxidized low-density lipoprotein ((-)OCl-LDL) has been shown to stimulate various functions of human polymorphonuclear leukocytes (PMNLs). Incubation of PMNLs with (-)OCl-LDL (produced by incubation of 0.4 mM LDL cholesterol with 1 mM NaOCl for 40 min at 37 degrees C) but not native or copper-oxidized LDL induced a substantial generation of reactive oxygen species (ROS) as measured by means of chemiluminescence with one peak at 10-12 min. Upon stimulation with (-)OCl-LDL about 70% of ROS (hydrogen peroxide and superoxide anion) were released from the cells into the extracellular environment. The (-)OCl-LDL-induced increase of the respiratory burst was dependent upon the dose, exposure time, and extent of LDL oxidation. Cytochalasin B, an inhibitor of phagocytosis, markedly diminished the LDL-induced ROS generation to nearly 40% of control values. (-)OCl-LDL enhanced the adhesion of PMNLs to human umbilical venous endothelial cells 2.5-fold as compared to native LDL and promoted the secretion of the active granule enzymes lysozyme and beta-glucuronidase. Together, the results suggest a potential role of LDL-activated PMNLs in initiating and/or maintaining the inflammatory process during the early phase of atheroscle...Continue Reading

References

Apr 1, 1976·Biochemical Medicine·K Yagi
Jan 1, 1992·The International Journal of Biochemistry·T StelmaszyńskaR J Schaur
Jun 22, 1992·Biochimica Et Biophysica Acta·W JessupR T Dean
Oct 10, 1986·Science·S J WeissS Regiani
Jul 1, 1988·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·J C KhooD Steinberg
Jul 1, 1995·Arteriosclerosis, Thrombosis, and Vascular Biology·E MalleG Waeg
Aug 1, 1994·Atherosclerosis·M S Penn, G M Chisolm
Nov 17, 1994·Biochimica Et Biophysica Acta·R MaebaN Ueta
Dec 1, 1994·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·E MaggiG Bellomo
Jul 1, 1994·The Journal of Clinical Investigation·A DaughertyJ W Heinecke
Jan 14, 1994·Clinica Chimica Acta; International Journal of Clinical Chemistry·W LeonhardtS Nitzsche
Sep 1, 1995·Bioscience, Biotechnology, and Biochemistry·B H RyuP Greenspan
Mar 15, 1996·The Journal of Clinical Investigation·L J HazellR Stocker

❮ Previous
Next ❯

Citations

Dec 23, 2003·The International Journal of Biochemistry & Cell Biology·Steffi KoppraschJürgen Grässler
Oct 6, 2000·Free Radical Biology & Medicine·Z D NightingaleG J Handelman
Oct 29, 2000·Free Radical Biology & Medicine·L J van TitsP H Willems
Nov 24, 1999·Micron : the International Research and Review Journal for Microscopy·K Löster, R Horstkorte
Dec 14, 2002·The International Journal of Biochemistry & Cell Biology·Pirkko J PussinenErnst Malle
Nov 25, 2003·Kidney International·Ernst MalleHermann-Josef Grone
Dec 17, 2005·Antioxidants & Redox Signaling·U Julius, J Pietzsch
Dec 23, 1999·Current Opinion in Hematology·C C WinterbournA J Kettle
Jun 5, 2003·American Journal of Respiratory Cell and Molecular Biology·Julie B SedgwickWilliam W Busse
Dec 12, 2001·Biochemical and Biophysical Research Communications·E MalleH J Gröne
Nov 17, 2007·Free Radical Biology & Medicine·Gary J DeverCorinne M Spickett
May 27, 2004·The American Journal of Pathology·Stefan PorubskyHermann-Josef Gröne
May 1, 1999·Experimental Neurology·J N KellerW R Markesbery
May 16, 2006·Biochimica Et Biophysica Acta·Ernst MalleMichael J Davies
Mar 19, 2014·Chemistry and Physics of Lipids·Alexej V SokolovOleg M Panasenko
Dec 3, 2014·Clinics in Chest Medicine·Renee D Stapleton, Benjamin T Suratt
Dec 24, 2008·Biochimica Et Biophysica Acta·Jeong Han KangOh-Shin Kwon
Sep 12, 2003·The Journal of Biological Chemistry·Gunther MarscheErnst Malle
Sep 24, 2004·Physiological Reviews·Roland Stocker, John F Keaney
Jun 9, 2001·Arteriosclerosis, Thrombosis, and Vascular Biology·M R McCallB Frei
Jul 15, 2000·Arteriosclerosis, Thrombosis, and Vascular Biology·A C CarrB Frei
Jul 5, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Tadashi HasegawaHartmut Jaeschke
Jul 16, 2013·Molecular and Cellular Biochemistry·Sarama SahaSteffi Kopprasch
Dec 17, 2008·Clinical Science·Fatiha Tabet, Kerry-Anne Rye
Jan 20, 2004·Journal of Biomedical Science·Riikka MäkeläTerho Lehtimäki

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

ApoE, Lipids & Cholesterol

Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.