Hypochlorous-Acid-Generating Electrochemical Scaffold for Treatment of Wound Biofilms

Scientific Reports
Mia Mae KiamcoHaluk Beyenal

Abstract

Biofilm formation causes prolonged wound infections due to the dense biofilm structure, differential gene regulation to combat stress, and production of extracellular polymeric substances. Acinetobacter baumannii, Staphylococcus aureus, and Pseudomonas aeruginosa are three difficult-to-treat biofilm-forming bacteria frequently found in wound infections. This work describes a novel wound dressing in the form of an electrochemical scaffold (e-scaffold) that generates controlled, low concentrations of hypochlorous acid (HOCl) suitable for killing biofilm communities without substantially damaging host tissue. Production of HOCl near the e-scaffold surface was verified by measuring its concentration using needle-type microelectrodes. E-scaffolds producing 17, 10 and 7 mM HOCl completely eradicated S. aureus, A. baumannii, and P. aeruginosa biofilms after 3 hours, 2 hours, and 1 hour, respectively. Cytotoxicity and histopathological assessment showed no discernible harm to host tissues when e-scaffolds were applied to explant biofilms. The described strategy may provide a novel antibiotic-free strategy for treating persistent biofilm-associated infections, such as wound infections.

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Citations

Jan 23, 2020·Antimicrobial Agents and Chemotherapy·Yash S RavalRobin Patel
Apr 7, 2020·Journal of Applied Microbiology·H M ZmudaH Beyenal
Oct 2, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·H Enis KarahanYuan Chen
Aug 17, 2020·Microorganisms·Waleska Stephanie da Cruz NizerJoerg Overhage
Jan 6, 2021·Antimicrobial Agents and Chemotherapy·Laure FlurinRobin Patel
Jan 14, 2021·Microorganisms·Pianpian YanDeog-Hwan Oh
Oct 12, 2019·ACS Applied Materials & Interfaces·Eloise Parry-NweyeTagbo H R Niepa
Jan 20, 2021·ACS Biomaterials Science & Engineering·Hao WangDacheng Ren
Jan 8, 2022·Veterinary Surgery : VS·Rachel HealdShaurya Prakash

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Methods Mentioned

BETA
scraping

Software Mentioned

Sigma Plot©
LabVIEW

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