Hypocretin-1 activates G proteins in arousal-related brainstem nuclei of rat

Neuroreport
René BernardHelen A Baghdoyan

Abstract

The hypocretin (hcrt) ligand-receptor system contributes to the maintenance of wakefulness. Hypocretin receptors are coupled to guanine nucleotide binding (G) proteins and the present study tested the hypothesis that hcrt-1 would activate G proteins in rat brainstem nuclei known to regulate arousal states. In vitro [35S]GTPgammaS autoradiography was performed using coronal brain stem sections from six rats. Hcrt-1 (200 nM) significantly increased [35S]GTPgammaS binding over basal levels in locus coeruleus (24%), dorsal raphe nucleus (12%), pontine reticular nucleus, oral part (28%), and pontine reticular nucleus, caudal part (40%). This is the first study to quantify and localize hcrt-1-induced G protein activation in specific brain stem nuclei. The finding that hcrt-1 stimulated [35S]GTPgammaS binding suggests that some hcrt receptors in pontine brain stem couple to inhibitory G proteins.

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Citations

Nov 12, 2003·Life Sciences·C Harrison, J R Traynor
Nov 25, 2005·Anesthesiology·Ralph Lydic, Helen A Baghdoyan
Jul 11, 2008·Brain Research·Heinrich S Gompf, Gary Aston-Jones
Aug 2, 2013·British Journal of Pharmacology·J P Kukkonen, C S Leonard
Jun 22, 2006·Physiology & Behavior·Catherine M Kotz
Jul 22, 2014·Medicinal Research Reviews·Quanhui ChenDong Gao
May 31, 2016·Biochemical and Biophysical Research Communications·Jyrki P Kukkonen
Nov 19, 2003·The European Journal of Neuroscience·René BernardHelen A Baghdoyan
Dec 22, 2004·The Journal of Biological Chemistry·Tomas HolmqvistJyrki P Kukkonen
Aug 3, 2002·Nature Reviews. Neuroscience·Edward F Pace-Schott, J Allan Hobson
Dec 28, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Catherine M KotzCharles J Billington
Oct 5, 2012·American Journal of Physiology. Cell Physiology·Jyrki P Kukkonen
Oct 4, 2013·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Jennifer A TeskeCatherine M Kotz

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