Hypomethylation and hypermethylation of the tandem repetitive 5S rRNA genes in Arabidopsis

The Plant Journal : for Cell and Molecular Biology
Isabelle VaillantSylvette Tourmente

Abstract

5S ribosomal DNA (5S rDNA) is organized in tandem repeats on chromosomes 3, 4 and 5 in Arabidopsis thaliana. One part of the 5S rDNA is located within the heterochromatic chromocenters, and the other fraction forms loops with euchromatic features that emanate from the chromocenters. We investigated whether the A. thaliana heterochromatin, and particularly the 5S rDNA, is modified when changing the culture conditions (cultivation in growth chamber versus greenhouse). Nuclei from challenged tissues displayed larger total, as well as 5S rDNA, heterochromatic fractions, and the DNA methyltransferase mutants met1 and cmt3 had different impacts in Arabidopsis. The enlarged fraction of heterochromatic 5S rDNA was observed, together with the reversal of the silencing of some 5S rRNA genes known as minor genes. We observed hypermethylation at CATG sites, and a concomitant DNA hypomethylation at CG/CXG sites in 5S rDNA. Our results show that the asymmetrical hypermethylation is correlated with the ageing of the plants, whereas hypomethylation results from the growth chamber/culture conditions. In spite of severely reduced DNA methylation, the met1 mutant revealed no increase in minor 5S rRNA transcripts in these conditions. The increasin...Continue Reading

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Citations

Dec 17, 2011·Plant & Cell Physiology·Elodie LayatSylvette Tourmente
Oct 11, 2008·Plant & Cell Physiology·Julien DouetSylvette Tourmente
Jul 12, 2018·Journal of Molecular Evolution·Y J Tamayo-OrdóñezL F Sánchez-Teyer

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