Hypoosmolar conditions reduce extracellular volume fraction and enhance epileptiform activity in the CA3 region of the immature rat hippocampus

Journal of Neuroscience Research
W KilbH J Luhmann

Abstract

The osmolarity of the extracellular space (ECS) compartment is an important factor determining the excitability of neuronal tissue. In the adult hippocampus an important role of osmolarity and ECS diffusion parameters on the susceptibility to epileptic events is well established, but the influence of hypo- and hyperosmolar conditions on the immature hippocampus remains elusive. To investigate the influence of osmolarity on epileptiform activity, extracellular field potentials were recorded in the CA3 region of hippocampal slices of immature (postnatal days 4-7) Wistar rats. The ECS diffusion parameters were determined by the real-time tetramethylammonium (TMA+) iontophoretic method with ion-selective microelectrodes in immature hippocampal slices and showed a lack of diffusion anisotropy; a tortuosity of about 1.39; and a volume fraction, alpha, of 0.41 +/- 0.01 (n = 10 slices). A reduction in osmolarity of -90 mOsm induced a decrease in alpha to 0.17 +/- 0.02 (n = 4 slices). The frequency of epileptiform activity elicited in 10-50 microM 4-AP-containing low-Mg2+ solution was increased under -90 mOsm and -40 mOsm hypoosmolar conditions by 39.9% +/- 8.1% (n = 16) and 24.1% +/- 4.8% (n = 10), respectively, whereas hyperosmolar so...Continue Reading

References

Nov 4, 1976·The New England Journal of Medicine·K B Nelson, J H Ellenberg
Sep 9, 1975·Pflügers Archiv : European journal of physiology·J DiMattioA Wald
Feb 1, 1991·Journal of Neurophysiology·H J Luhmann, D A Prince
Sep 1, 1991·American Journal of Diseases of Children·J P KeatingP R Dodge
Jan 1, 1991·Journal of the Neurological Sciences·R D Andrew
Aug 10, 1990·Science·C J McBainR Dingledine
May 1, 1989·Journal of Neurophysiology·S F Traynelis, R Dingledine
Sep 25, 1989·Brain Research·R D AndrewA S Rosen
Jan 1, 1987·Epilepsia·S L Moshé
Jan 1, 1986·Advances in Experimental Medicine and Biology·U Heinemann
Jan 1, 1993·Journal of Neurophysiology·E C Burgard, J J Hablitz
Jun 1, 1993·Journal of Neurophysiology·V Saly, R D Andrew
Jun 14, 1996·Brain Research. Developmental Brain Research·C Psarropoulou, M Avoli
Feb 1, 1997·The Journal of Physiology·S C BarabanP A Schwartzkroin
Apr 18, 1998·Journal of Neurophysiology·S C Baraban, P A Schwartzkroin
Jun 19, 1998·Neuroreport·T MazelE Syková
Oct 7, 1998·Epilepsy Research·P A SchwartzkroinD W Hochman
Dec 14, 1999·European Journal of Pharmacology·A SabauA Draguhn
Mar 25, 2000·Neurochemistry International·E Syková, A Chvátal
Jul 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·K C Chen, C Nicholson
Dec 1, 2000·Progress in Brain Research·E SykováS Prokopová-Kubinová
Dec 7, 2000·Mental Retardation and Developmental Disabilities Research Reviews·J W Swann, J J Hablitz
Sep 7, 2002·Mental Retardation and Developmental Disabilities Research Reviews·Linda D Cowan
Oct 10, 2002·The European Journal of Neuroscience·P P QuilichiniH Gozlan
Feb 10, 2004·Proceedings of the National Academy of Sciences of the United States of America·Richard PietStéphane H R Oliet
Mar 24, 2004·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Eric A BushongMark H Ellisman
Jan 26, 2005·Trends in Neurosciences·Rosa CossartYehezkel Ben-Ari
Feb 25, 2005·Journal of Neurophysiology·Michael M Haglund, Daryl W Hochman

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Citations

Aug 12, 2010·Analytical Chemistry·Amy E HamsherStephen G Weber
Mar 6, 2009·Epilepsia·Andréy MazaratiRaman Sankar
Oct 17, 2008·Physiological Reviews·Eva Syková, Charles Nicholson
May 23, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Xiaoming YaoGeoffrey T Manley
Sep 17, 2014·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Lýdia Vargová, Eva Syková
May 4, 2013·Journal of Neuroscience Methods·Klaus AlbusRichard Kovács
Mar 21, 2009·Clinical Neurophysiology : Official Journal of the International Federation of Clinical Neurophysiology·E ShaharP L Carlen
Apr 28, 2012·The European Journal of Neuroscience·Josef ZamecnikLydia Vargova
Apr 8, 2011·Trends in Neurosciences·Dmitri A RusakovJeremy M Henley
May 22, 2007·Neurochemistry International·Eva Syková, Lýdia Vargová
Aug 6, 2017·Reviews in the Neurosciences·Katherine L PerkinsSabina Hrabetova
Feb 27, 2008·Journal of Cellular Physiology·Rodrigo FrancoLenin D Ochoa de la Paz
Oct 6, 2017·Frontiers in Cellular Neuroscience·Thomas R MurphyTodd A Fiacco
Jul 19, 2018·Frontiers in Pharmacology·Daria VinokurovaGuzel Valeeva
Mar 18, 2019·Neurochemical Research·Robert ColbournSabina Hrabetova
Dec 21, 2019·Neurochemical Research·N Joan AbbottAlexei Verkhratsky
Nov 13, 2020·Frontiers in Neuroscience·Federico N SoriaJan Tønnesen

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