PMID: 8938699Oct 1, 1996Paper

Hypoosmotic shocks induce elevation of resting calcium level in Duchenne muscular dystrophy myotubes contracting in vitro

Neuromuscular Disorders : NMD
N ImbertG Raymond

Abstract

In Duchenne muscular dystrophy (DMD) muscle cells which lack dystrophin, contraction seems to be a dominant factor contributing to the abnormal elevated intracellular calcium level. Human normal and DMD contracting myotubes cocultured with nervous cells were exposed to a hypotonic medium to mimic contraction-induced mechanical stress on the membrane, and the cytoplasmic calcium activity was simultaneously monitored (Indo-1). Hypotonic shocks induced a reversible [Ca2+]i increase in 81% of the DMD cells vs. 54% of control. In addition, responses were qualitatively different: most of DMD myotubes displayed a fast increase of Ca2+ flowing from the edge of the myotube while the response in normal cells was slow and diffuse. The fact that these responses were not affected by ryanodine, was in favour of an external source of Ca2+ involved in the hypoosmotic shocks. The localized increase of Ca2+ in DMD myotubes, inhibited by Gd3+, could result from sites of high mechanosensitive channel activity or density which could constitute a pathway for Ca2+ entry provided these cells contract.

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Citations

Jul 1, 2002·Indian Journal of Clinical Biochemistry : IJCB·Harleen MangatMp Js Anandaraj
Jan 8, 1999·Journal of the Neurological Sciences·M H DisatnikT A Rando
Apr 18, 2002·Neuroscience Letters·Clarisse VandebrouckChristian Cognard
Feb 13, 2001·Neuromuscular Disorders : NMD·C VandebrouckG Raymond
Sep 11, 2002·Neuromuscular Disorders : NMD·Urs T RueggTimo M Buetler
Jan 1, 2012·BoneKEy Reports·Victor H MorgenrothPaula R Clemens
Oct 9, 2009·The Journal of Biological Chemistry·Jessica SabourinBruno Constantin
Jun 23, 2006·Clinical and Experimental Pharmacology & Physiology·Jeffry B Lansman, Alfredo Franco-Obregón
Dec 4, 2009·Clinical Neurophysiology : Official Journal of the International Federation of Clinical Neurophysiology·Javier RodríguezArmando Malanda
May 11, 2010·Journal of Electromyography and Kinesiology : Official Journal of the International Society of Electrophysiological Kinesiology·Javier RodríguezArmando Malanda
Sep 18, 2002·The Journal of Cell Biology·Clarisse VandebrouckPhilippe Gailly
Aug 10, 2006·Journal of Muscle Research and Cell Motility·Bruno ConstantinChristian Cognard
Mar 3, 2010·Journal of Muscle Research and Cell Motility·J SabourinBruno Constantin
Mar 28, 2002·Physiological Reviews·Derek J BlakeKay E Davies
Apr 5, 2020·Cells·Jun Hee ChoiEun Hui Lee
Apr 21, 2001·Cardiovascular Drug Reviews·L C AddingL E Gustafsson
Mar 28, 2002·The American Journal of Clinical Nutrition·Timo M BuetlerUrs T Ruegg

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