Hypophosphorylation of the RB protein in S and G2 as well as G1 during growth arrest

Experimental Cell Research
A Yen, R Sturgill

Abstract

The RB tumor suppressor protein is a cell cycle regulator, where hypophosphorylated RB is associated with G1/0 arrest and its cyclin-dependent phosphorylation in G1 allows progression from G1 to S. The present report shows that in human leukemia cells induced to undergo growth arrest with sodium butyrate or DMSO, hypophosphorylation of the RB protein is not G1 restricted and also occurs in S and G2/M cells as well as in G1 cells when growth is inhibited. While all of the RB protein in G1/0 cells is hypophosphorylated, residual cells in S and G2 have significant detectable amounts of hypophosphorylated RB as well as still hyperphosphorylated RB protein. Thus RB hypophosphorylation can be induced in S and G2 as well as the G1 phase. The results show that growth retardation in other than the G1 phase is associated with occurrence of hypophosphorylated RB. RB may thus have a broader capability to inhibit proliferation than just in G1.

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Citations

Apr 2, 2010·The Journal of Biological Chemistry·Alex James FulcherDavid A Jans
May 29, 2000·Molecular and Cellular Biology·P M FlattJ A Pietenpol
Feb 7, 2001·Molecular and Cellular Biology·V GottifrediC Prives
Nov 16, 2004·Veterinary Immunology and Immunopathology·M Julia B F FlaminioDouglas F Antczak
Oct 16, 2009·American Journal of Physiology. Renal Physiology·Valerie A LuyckxThomas F Mueller
Feb 24, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Josep Maria RoigAdela Mazo
Oct 20, 2004·Cancer Research·Checo J Rorie, Bernard E Weissman

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