PMID: 6172271Nov 1, 1981Paper

Hyporesponsiveness of canine bronchoalveolar lymphocytes to mitogens: II. Analysis of macromolecular synthesis, T-cell antigen, and mitogen-binding

Experimental Lung Research
M J Ansfield, H B Kaltreider

Abstract

Canine bronchoalveolar lymphocytes (BAL) and peripheral blood lymphocytes (PBL) were prepared by filtration over nylon wool columns and were studied for their ability to: (a) synthesize protein, RNA, and DNA after mitogen stimulation; (b) express T-cell antigen; and (c) bind radiolabeled mitogen. Bronchoalveolar lymphocytes were shown to be markedly hyporesponsive to mitogen stimulation as assessed by DNA synthesis after 72 hr of culture or by protein and RNA synthesis after 24 hr of culture. The hyporesponsiveness of BAL was not due to a decrease in the number of T-cells; results of an indirect immunofluorescent assay employing a rabbit antiserum indicated similar percentages of T-cells in BAL (78%) and PBL (79%). The hyporesponsiveness was not due to abnormalities in mitogen binding by BAL; results of a radiolabeled mitogen-binding assay indicated essentially no difference in mitogen receptor number or affinity between BAL and PBL. Thus, the initial interaction bronchoalveolar T lymphocytes and mitogen appears to be normal. This normal interaction with mitogen, however, fails to trigger an appropriate level of macromolecular synthesis by BAL. This suggests that the hyporesponsiveness of BAL is due to an abnormality in one of ...Continue Reading

References

Sep 1, 1975·Cortex; a Journal Devoted to the Study of the Nervous System and Behavior·L S Rosenthal, P Fedio
Dec 1, 1972·The Journal of Clinical Investigation·S BoldtS Kornfeld
Oct 14, 1971·Biochimica Et Biophysica Acta·J E KayM Atkins
Dec 30, 1969·Biochimica Et Biophysica Acta·S Kornfeld
Mar 8, 1965·Biochimica Et Biophysica Acta·T L STECK, D F HOELZLWALLACH

❮ Previous
Next ❯

Citations

May 15, 2001·American Journal of Respiratory Cell and Molecular Biology·J R WrightR J Folz
Jan 1, 1982·American Journal of Industrial Medicine·H Haubenstock, J G Bekesi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.