Hyposmotic shock stimulates insulin secretion by two distinct mechanisms. Studies with the betaHC9 cell

American Journal of Physiology. Endocrinology and Metabolism
Susanne G StraubGeoffrey W G Sharp

Abstract

Exposure of betaHC9 cells to a Krebs-Ringer bicarbonate-HEPES buffer (KRBH) made hypotonic by a reduction of 25 mM NaCl resulted in a prompt stimulation of insulin release. The stimulation was transient, and release rates returned to basal levels after 10 min. The response resembles that of the first phase of glucose-stimulated insulin release. The response did not occur if the reduction in NaCl was compensated for by the addition of an equivalent osmolar amount of sorbitol, so the stimulation of release was due to the osmolarity change and not the reduction in NaCl. The hyposmotic shock released insulin in KRBH with or without Ca(2+). The L-type Ca(2+) channel blocker nitrendipine inhibited the response in normal KRBH but had no effect in KRBH without Ca(2+) despite the latter response being larger than in the presence of extracellular Ca(2+). Similar data were obtained with calciseptine, which also blocks L-type channels. The T-type Ca(2+) channel blocker flunarizine was without effect, as was the chloride channel blocker DIDS. In parallel studies, the readily releasable pool of insulin-containing granules was monitored. Immunoprecipitation of the target-SNARE protein syntaxin and co-immunoprecipitation of the vesicle-SNARE V...Continue Reading

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Citations

Nov 10, 2009·Biochemical and Biophysical Research Communications·Yu-Hsiang Lee, Ching-An Peng
Oct 4, 2008·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Marian TomasiakPiotr Radziwon
Jan 7, 2011·Molecular Biology of the Cell·Elisabeth T BarfodSteven D Lidofsky
Aug 18, 2006·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Martin JakabMarkus Ritter
Jan 24, 2007·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Christian AmatoreYann Verchier
Feb 27, 2008·Journal of Cellular Physiology·Rodrigo FrancoLenin D Ochoa de la Paz
Jan 17, 2019·American Journal of Physiology. Cell Physiology·Toshiaki SawataniTomohisa Ishikawa
Aug 27, 2005·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Zuzana BacováVladimir Strbák
Jul 11, 2006·American Journal of Physiology. Cell Physiology·Miki TakiiKoichi Nakayama
Nov 16, 2019·Scientific Reports·Vijayalakshmi DeivasikamaniPiruthivi Sukumar
Dec 20, 2011·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Vladimír Strbák
May 3, 2012·American Journal of Physiology. Endocrinology and Metabolism·M WillenborgI Rustenbeck

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