Hypothermia broadens the therapeutic time window of mesenchymal stem cell transplantation for severe neonatal hypoxic ischemic encephalopathy

Scientific Reports
So Yoon AhnWon Soon Park

Abstract

Recently, we have demonstrated that concurrent hypothermia and mesenchymal stem cells (MSCs) transplantation synergistically improved severe neonatal hypoxic ischemic encephalopathy (HIE). The current study was designed to determine whether hypothermia could extend the therapeutic time window of MSC transplantation for severe neonatal HIE. To induce HIE, newborn rat pups were exposed to 8% oxygen for 2 h following unilateral carotid artery ligation on postnatal day (P) 7. After approving severe HIE involving >50% of the ipsilateral hemisphere volume, hypothermia (32 °C) for 2 days was started. MSCs were transplanted 2 days after HIE modeling. Follow-up brain MRI, sensorimotor function tests, assessment of inflammatory cytokines in the cerebrospinal fluid (CSF), and histological evaluation of peri-infarction area were performed. HIE induced progressively increasing brain infarction area over time, increased cell death, reactive gliosis and brain inflammation, and impaired sensorimotor function. All these damages observed in severe HIE showed better, robust improvement with a combination treatment of hypothermia and delayed MSC transplantation than with either stand-alone therapy. Hypothermia itself did not significantly reduce b...Continue Reading

References

Aug 1, 1985·Developmental Medicine and Child Neurology·C Robertson, N Finer
Nov 1, 1995·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·W D DietrichR Busto
Oct 1, 1993·Journal of Neurochemistry·G D Zeevalk, W J Nicklas
Jan 1, 1996·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·H Y KilC A Piantadosi
Dec 26, 1995·Biochemical and Biophysical Research Communications·A D EdwardsH Mehmet
Mar 21, 2000·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·J GuanP D Gluckman
May 23, 2002·Journal of Studies on Alcohol. Supplement·Linda Patia Spear
Jun 10, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Masaharu Sakoh, Albert Gjedde
Sep 27, 2005·Brain & Development·Hany AlyBothina M Hasanein
Oct 14, 2005·The New England Journal of Medicine·Seetha ShankaranUNKNOWN National Institute of Child Health and Human Development Neonatal Research Network
Apr 15, 2006·Journal of Korean Medical Science·Won Soon ParkMunhyang Lee
Oct 19, 2007·The Cochrane Database of Systematic Reviews·S JacobsP Davis
Jun 25, 2008·Journal of Translational Medicine·Jesse T Lang, Louise D McCullough
Jul 29, 2008·Stroke; a Journal of Cerebral Circulation·Midori YenariMiguel Perez-Pinzon
Oct 3, 2009·The New England Journal of Medicine·Denis V AzzopardiUNKNOWN TOBY Study Group
Jul 26, 2011·American Journal of Obstetrics and Gynecology·Christopher S EnnenErnest M Graham
Mar 21, 2012·Pediatric Research·Cindy T J van VelthovenCobi J Heijnen
Jan 5, 2013·Stroke; a Journal of Cerebral Circulation·So Yoon AhnWon Soon Park
Jan 7, 2014·The Journal of Pediatrics·C Michael CottenJoanne Kurtzberg
Feb 11, 2014·The Journal of Pediatrics·Yun Sil ChangWon Soon Park
Feb 12, 2014·Klinische Pädiatrie·L Gortner
Jun 3, 2016·Neonatology·So Yoon AhnWon Soon Park

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Citations

Feb 23, 2020·Current Stem Cell Research & Therapy·Ifrah AnwarZeeshan Shaukat
Apr 2, 2020·Current Stem Cell Research & Therapy·Xiaojie BaiHong Zhang
Apr 4, 2021·International Journal of Molecular Sciences·Inês SerrenhoGraça Baltazar
Jan 17, 2021·Pediatrics International : Official Journal of the Japan Pediatric Society·Yoshiaki Sato, Masahiro Tsuji
Jun 23, 2021·Seminars in Fetal & Neonatal Medicine·Aravanan A ChakkarapaniUNKNOWN Newborn Brain Society Guidelines and Publications Committee
Aug 1, 2021·Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·Fei ChenZhi-You Cai
Nov 14, 2021·Journal of Neuroinflammation·Marina SeitzJosephine Herz

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay

Clinical Trials Mentioned

NCT02274428

Software Mentioned

ImageJ
SPSS

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