Hypothermic Storage of Human Umbilical Vein Endothelial Cells and Their Hydrogel Constructs.

Biopreservation and Biobanking
Xiaozhang ZhangGang Zhao

Abstract

Human umbilical vein endothelial cells (HUVECs) have great potential in tissue engineering, regenerative medicine, and clinical applications. There is an ever-increasing demand to provide living HUVECs and HUVECs-hydrogel constructs to end users when needed in cell-based therapy and clinical applications. However, current methods to provide living cells and their constructs are mainly continuous culture and cryopreservation, which are high-cost, labor-intensive, time-consuming and inflexible. The research about hypothermic storage of HUVECs and their hydrogel constructs is still limited. Here, we studied the cell survival of HUVECs without encapsulation (W/O Encap) or with encapsulation (alginate, alginate with carboxymethyl chitosan [CMCH]) at 4°C and 25°C during 7 days, respectively. Also, we explored the optimal CMCH concentration for hypothermic storage, which is 0.5% (w/v) at 4°C and 25°C. Moreover, we evaluated the cell attachment after hypothermic storage. Our results enable the hypothermic storage of HUVECs and HUVEC-hydrogel constructs, and facilitate their application in tissue engineering and clinical medicine.

References

Jun 4, 2008·Cancer Research·Reza IzadpanahBruce A Bunnell
Aug 17, 2012·Arteriosclerosis, Thrombosis, and Vascular Biology·Peter Libby
Dec 1, 2011·Biopreservation and Biobanking·Hart StadnickJelena Lecak Holovati
Jan 31, 2016·Stem Cells Translational Medicine·Stephen SwiokloChe J Connon
Feb 20, 2016·Circulation Research·Michael A Gimbrone, Guillermo García-Cardeña
Aug 15, 2017·Biopreservation and Biobanking·Harriëtte OldenhofWillem F Wolkers
Jan 25, 2018·Biopreservation and Biobanking·Maksim V PuzanovRenata I Dmitrieva
Jul 17, 2018·Biopreservation and Biobanking·Luciana Da Silveira CavalcanteJelena L Holovati

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BETA
Fluorescence

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Pro Plus

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