PMID: 8972386Dec 1, 1996Paper

Hypothesis for a serine proteinase-like domain at the COOH terminus of Slowpoke calcium-activated potassium channels

The Journal of General Physiology
G W MossE Moczydlowski

Abstract

Bovine pancreatic trypsin inhibitor (BPTI) is a 58-residue protein with three disulfide bonds that belongs to the Kunitz family of serine proteinase inhibitors. BPTI is an extremely potent inhibitor of trypsin, but it also specifically binds to various active and inactive serine proteinase homologs with KD values that range over eight orders of magnitude. We previously described an interaction of BPTI at an intracellular site that results in the production of discrete subconductance events in large conductance Ca2+ activated K+ channels (Moss, G.W.J., and E. Moczydlowski. 1996, J. Gen. Physiol, 107:47-68). In this paper, we summarize a variety of accumulated evidence which suggests that BPTI binds to a site on the KCa channel protein that structurally resembles a serine proteinase. One line of evidence includes the finding that the complex of BPTI and trypsin, in which the inhibitory loop of BPTI is masked by interaction with trypsin, is completely ineffective in the production of substate events in the KCa channel. To further investigate this notion, we performed a sequence analysis of the alpha-subunit of cloned slowpoke KCa channels from Drosophila and mammals. This analysis suggests that a region of approximately 250 residu...Continue Reading

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Citations

Feb 2, 1999·Clinical Reviews in Allergy & Immunology·H S Margolius
Dec 23, 2008·Cellular and Molecular Life Sciences : CMLS·J CuiU S Lee
Nov 7, 2001·The Journal of General Physiology·J Shi, J Cui
Feb 5, 2003·The Journal of General Physiology·Karl L Magleby
Nov 2, 2005·The Journal of General Physiology·Teresa GiraldezFred J Sigworth
Jun 1, 2006·The Journal of General Physiology·Anthony A Fodor, Richard W Aldrich
Mar 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·S BianE Moczydlowski
Dec 9, 2014·Channels·Zoltan TakacsEdward G Moczydlowski
May 13, 1999·Nature Neuroscience·M SchreiberL Salkoff

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