PMID: 8941517Nov 1, 1996Paper

Hypothyroidism alters diaphragm muscle development

Journal of Applied Physiology
G C SieckW Z Zhan

Abstract

The impact of hypothyroidism (Hyp) on myosin heavy chain (MHC) isoform expression, maximum specific force (P0), fatigability, and maximum unloaded shortening velocity (V0) was determined in the rat diaphragm muscle (Dia) at 0, 7, 14, 21, and 28 days of age. Hyp was induced by treating pregnant rats with 6-n-propyl-2-thiouracil (0.05% in drinking water) beginning at gestational day 10 and was confirmed by reduced plasma levels of 3,5,3'-triiodothyronine and thyroxine. MHC isoforms were separated on sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels and analyzed by densitometry. Isometric P0 and fatigue resistance of the Dia were measured in vitro at 26 degrees C, and V0 was determined at 15 degrees C with the slack test. Compared with control muscles, expression of MHC-slow was higher and expression of adult fast MHC isoforms was lower in Hyp Dia at all ages. The neonatal isoform of MHC continued to be expressed in the Hyp Dia until day 28. At each age, P0 and fatigability were reduced and V0 was slower in the Hyp Dia. We conclude that Hyp-induced alterations in MHC isoform expression do not fully predict the changes in Dia contractile properties.

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Citations

Jul 29, 2006·Journal of Applied Physiology·Paige C GeigerGary C Sieck
Jan 12, 2008·Journal of Applied Physiology·Carlos B MantillaGary C Sieck
Feb 22, 2001·Journal of Applied Physiology·G C Sieck, M Regnier
Apr 17, 2004·Acta Physiologica Scandinavica·D E T O'Neill, E G Noble
May 28, 2013·Experimental Physiology·R A O'ConnellK D O'Halloran
Jun 9, 2007·Journal of Applied Physiology·Gary C SieckY S Prakash
Feb 4, 2003·Journal of Applied Physiology·Gary C SieckWen-Zhi Zhan
May 9, 1998·Journal of Applied Physiology·W Z ZhanG C Sieck
Aug 31, 2020·Drug Discovery Today. Disease Models·Matthew J Fogarty, Gary C Sieck
Jan 29, 2021·Journal of Applied Physiology·Matthew J FogartyGary C Sieck
Sep 12, 1998·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·J F WatchkoG C Sieck

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