Hypotonic Ca2+ signaling and volume regulation in proliferating and quiescent cells from multicellular spheroids

Journal of Cellular Physiology
H SauerM Wartenberg

Abstract

Hypotonicity-induced Ca2+ signals and volume regulation were studied in proliferating and quiescent subpopulations of multicellular prostate cancer spheroids. Enzymatic dissociation of multicellular spheroids 100+/-19 microm in diameter, which are entirely proliferative, yielded a population of cells with a mean cell diameter of 17.5+/-1.4 microm. After dissociation of spheroids in a size class of 200+/-30, 300+/-60, and 400+/-65 microm in diameter, two subpopulations of cells with mean cell diameters corresponding to 12.9+/-1.9 microm and 16.7+/-2 microm were discriminated. The subpopulation of large cells was shown to be proliferative by positive Ki-67 antibody staining; the subpopulation of small cells was Ki-67 negative, indicating cell quiescence. In a spheroid size class of 100+/-19 microm, a distinct subpopulation of quiescent cells was absent. Superfusion by hypotonic solutions revealed that only the proliferating cell fraction showed a regulatory volume decrease (RVD) and a [Ca2+]i transient. Both effects were absent in the quiescent cell population. The [Ca2+]i transient persisted in low (10 nM) Ca2+ solution and in the presence of 4 mM extracellular Ni2+ but was abolished in the presence of the endoplasmic reticulum ...Continue Reading

References

Oct 1, 1992·Physiological Reviews·N A McCarty, R G O'Neil
Oct 1, 1992·Journal of Cellular Physiology·A Bredel-GeisslerW Mueller-Klieser
May 23, 1991·European Journal of Biochemistry·S vom DahlD Häussinger
May 1, 1990·Cell and Tissue Kinetics·R E Durand
Jan 1, 1985·The American Journal of Physiology·J K Foskett, K R Spring
May 15, 1983·International Journal of Cancer. Journal International Du Cancer·J CarlssonV P Collins
Jan 1, 1995·International Review of Cytology·E K Hoffmann, P B Dunham
Jun 1, 1995·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M J Berridge
Apr 1, 1993·The Journal of Membrane Biology·M CornetH A Kolb
Feb 1, 1995·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·G MazzottiN M Maraldi
Apr 1, 1995·Journal of Cellular Physiology·G P DowneyC K Chan
Jun 1, 1994·Cancer Metastasis Reviews·P L Olive, R E Durand
Aug 23, 1993·Biochimica Et Biophysica Acta·R MüllerF C Lucibello
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·H KobayashiR S Kerbel
Dec 1, 1995·The Journal of Cell Biology·F CastellinoA Luini
Jan 1, 1995·Micron : the International Research and Review Journal for Microscopy·M Wartenberg, H Acker
Jun 1, 1996·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·O BussolatiG C Gazzola
Jul 1, 1996·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·P E BallmerP J Garlick
Jan 1, 1988·Meat Science·S D Jones, W M Robertson

❮ Previous
Next ❯

Citations

Mar 20, 1998·International Journal of Cancer. Journal International Du Cancer·M WartenbergH Sauer
Jun 30, 2009·Journal of Cellular Biochemistry·J C Gallardo-PérezV Maldonado
Jul 6, 2004·Journal of Theoretical Biology·T AlarcónP K Maini
Dec 1, 2012·Biochimica Et Biophysica Acta·Edna Ayerim Mandujano-TinocoSara Rodríguez-Enríquez
Jul 11, 2006·Journal of Cellular Physiology·Klaus Lange, Joachim Gartzke
Jan 8, 2009·Physiological Reviews·Else K HoffmannStine F Pedersen
Sep 20, 2020·International Journal of Molecular Sciences·Fabrizio FontanaPatrizia Limonta
Sep 30, 1999·Experimental Cell Research·S F PedersenE K Hoffmann

❮ Previous
Next ❯

Related Concepts

Related Feeds

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.