PMID: 1195346Jan 1, 1975Paper

Hypotonic hemolysis of human red blood cells: a two-phase process

The Journal of Membrane Biology
J T Saari, J S Beck

Abstract

Previous use of hemolysis time measurement to determine permeability coefficients for the red blood cell membrane rested on the assumption that cells swelling in a hypotonic medium hemolyzed immediately on reaching critical volume. By preswelling red cells to various volumes prior to immersion in hemolytic solutions we extrapolate to the hemolysis time of red cells immersed at critical volume and thereby find a significant period of time during which the cells apparently remain in a spherical form prior to release of hemoglobin. Revised estimates of permeability coefficients follow from including this spherical (nonswelling) phase. In addition, the appreciation of a characteristic time period during which the membrane is under tension provides new opportunity to study physical and chemical properties of the membrane.

References

Dec 15, 1973·Molecular and Cellular Biochemistry·G Schwoch, H Passow
Oct 1, 1972·Microvascular Research·E Evans, Y C Fung
Dec 29, 1972·The Journal of Membrane Biology·M Poznansky, A K Solomon
Feb 27, 1973·Biochimica Et Biophysica Acta·C Y JungC J Balzer
Apr 25, 1973·Biochimica Et Biophysica Acta·C M ColleyL L van Deenen
Jan 1, 1974·Biophysical Journal·J T Saari, J S Beck
Jun 1, 1970·Canadian Journal of Physiology and Pharmacology·P B Canham, D R Parkinson
Nov 1, 1967·Journal of Theoretical Biology·J A Johnson, T A Wilson
Feb 1, 1958·Biochimica Et Biophysica Acta·O KEDEM, A KATCHALSKY
Sep 1, 1960·The Journal of General Physiology·D A GOLDSTEIN, A K SOLOMON
Apr 1, 1961·Journal of Cellular and Comparative Physiology·D DANON
Jan 1, 1959·International Review of Cytology·D A DICK
Apr 6, 1963·Nature·P C DODWELL, G R ENGEL

❮ Previous
Next ❯

Citations

Jan 1, 1988·Progress in Biophysics and Molecular Biology·G Benga
Jan 1, 1981·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·G Bartosz, W Leyko
Jul 1, 2005·Annals of Emergency Medicine·Robert SidmanGregory Jay
Sep 21, 2017·European Biophysics Journal : EBJ·Duobiao GeWeiping Ding
Sep 1, 1979·Journal of Neurosurgery·A R HudsonB R Bratton
Jan 17, 2012·Analytical Chemistry·Jeffrey R SooHooGlenn M Walker

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.