Hypotonic stress upregulates β- and γ-ENaC expression through suppression of ERK by inducing MKP-1.

American Journal of Physiology. Renal Physiology
N NiisatoY Marunaka

Abstract

We investigated a physiological role for ERK, a member of the MAPK family, in the hypotonic stimulation of epithelial Na(+) channel (ENaC)-mediated Na(+) reabsorption in renal epithelial A6 cells. We show that hypotonic stress causes a major dephosphorylation of ERK following a rapid transient phosphorylation. PD98059 (a MEK inhibitor) increases dephosphorylated ERK and enhances the hypotonic-stress-stimulated Na(+) reabsorption. ERK dephosphorylation is mediated by MAPK phosphatase (MKP). Hypotonic stress activates p38, which in turn induces MKP-1 and to a lesser extent MKP-3 mRNA expression. Inhibition of p38 suppresses MKP-1 induction, preventing hypotonic stress from dephosphorylating ERK. Inhibition of MKP-1 and -3 by the inhibitor NSC95397 also suppresses the hypotonicity-induced dephosphorylation of ERK. NSC95397 reduces both β- and γ-ENaC mRNA expression and ENaC-mediated Na(+) reabsorption stimulated by hypotonic stress. In contrast, pretreatment with PD98059 significantly enhances mRNA and protein expression of β- and γ-ENaC even under isotonic conditions. However, PD98059 only stimulates Na(+) reabsorption in response to hypotonic stress, suggesting that ERK inactivation by itself (i.e., under isotonic conditions) is...Continue Reading

References

Feb 1, 1994·Current Opinion in Genetics & Development·C J Marshall
Dec 22, 1999·Biochemical and Biophysical Research Communications·N NiisatoY Marunaka
Jun 1, 2000·The Journal of Biological Chemistry·J D StockandD C Eaton
Sep 21, 2000·Seminars in Immunology·M Saxena, T Mustelin
Mar 30, 2001·The Journal of Biological Chemistry·B MalikD C Eaton
Jan 24, 2003·American Journal of Physiology. Renal Physiology·Rachell E Booth, James D Stockand
Mar 22, 2003·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·David Pearce
Aug 5, 2004·American Journal of Physiology. Renal Physiology·Naomi NiisatoYoshinori Marunaka
Nov 3, 2006·American Journal of Respiratory Cell and Molecular Biology·Makoto YasudaYoshinori Marunaka
Mar 9, 2007·American Journal of Physiology. Renal Physiology·Akiyuki TarunoYoshinori Marunaka
Apr 3, 2007·Biochemical and Biophysical Research Communications·Naomi NiisatoYoshinori Marunaka
May 18, 2007·Biochemical and Biophysical Research Communications·Naomi NiisatoYoshinori Marunaka
Jul 31, 2007·Biochemical and Biophysical Research Communications·Naomi NiisatoYoshinori Marunaka
Sep 4, 2007·Biochemical and Biophysical Research Communications·Makoto YasudaYoshinori Marunaka
Oct 26, 2007·American Journal of Physiology. Renal Physiology·Akiyuki TarunoYoshinori Marunaka
Oct 4, 2008·Annual Review of Physiology·Douglas C EatonLucky Jain
Mar 3, 2009·American Journal of Respiratory Cell and Molecular Biology·Junji AsanoYoshinori Marunaka
Mar 17, 2009·Biochemical and Biophysical Research Communications·Takahiro YamadaYoshinori Marunaka
Mar 20, 2009·American Journal of Physiology. Renal Physiology·Lian LiuDouglas C Eaton
Apr 30, 2009·The Journal of Biological Chemistry·Thomas R KleymanRebecca P Hughey
Jun 16, 2009·Cell Biology International·Shinsaku TokudaYoshinori Marunaka
Oct 30, 2009·Current Opinion in Nephrology and Hypertension·Christopher J PasseroThomas R Kleyman
Nov 3, 2009·Biochemical and Biophysical Research Communications·Shinsaku TokudaYoshinori Marunaka
Feb 9, 2010·Biochemical and Biophysical Research Communications·Shinsaku TokudaYoshinori Marunaka
Feb 18, 2010·Proceedings of the American Thoracic Society·Douglas C EatonLucky Jain
Jul 16, 2010·American Journal of Physiology. Renal Physiology·Christopher J PasseroThomas R Kleyman
Jan 12, 2011·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Akiyuki Taruno, Yoshinori Marunaka
Oct 14, 2011·American Journal of Physiology. Renal Physiology·Christopher J PasseroThomas R Kleyman
Oct 27, 2011·Journal of Biomedicine & Biotechnology·Yoshinori MarunakaTakahiro Kubota

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Citations

Mar 7, 2014·Respiratory Physiology & Neurobiology·Yoshinori Marunaka
Dec 15, 2015·Inflammatory Bowel Diseases·Christian BarmeyerJörg-Dieter Schulzke
Apr 15, 2014·Journal of Cellular and Molecular Medicine·Shigekuni HosogiYoshinori Marunaka
Jul 13, 2016·BioMed Research International·Siguang XuXiumin Li
Jul 13, 2014·American Journal of Physiology. Lung Cellular and Molecular Physiology·Amity F EatonHui-Fang Bao
Oct 8, 2013·American Journal of Physiology. Lung Cellular and Molecular Physiology·Megan M GreenleeDouglas C Eaton
Aug 26, 2020·International Journal of Immunopathology and Pharmacology·Young Sun ChungKwang Won Jeong
Jan 1, 2014·Molecular and Cellular Therapies·Yoshinori MarunakaHiroshi Ikegaya

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