Hypotonically induced whole-cell currents in A6 cells: relationship with cell volume and cytoplasmic Ca2+

The Japanese Journal of Physiology
W G Yu, Masahiro Sokabe

Abstract

We investigated changes in whole-cell currents, cell volume, and intracellular calcium concentration ([Ca2+]i) during hypotonic stimulation in whole-cell clamped cultured amphibian renal cells (A6 cells). Upon being exposed to hypotonic solution (80% osmolality), the A6 cells swelled and peaked in the first 5 min, which was followed by a progressive decrease in cell volume termed regulatory volume decrease (RVD). Following the cell swelling, there were large increases in both outward- and inward-currents, which seemed to be carried by K+ efflux and Cl- efflux, respectively. A K+ channel blocker (TEA or quinine) or a Cl- channel blocker (NPPB or SITS) significantly inhibited both currents and RVD, suggesting that the inward- and outward-currents are highly correlated with each other and essential to RVD. Hypotonic stimulation also induced a transient [Ca2+]i increase, of which the time course was essentially similar to that of the currents. When internal and external Ca2+ were deprived to eliminate the Ca2+ transient increase, whole-cell currents and RVD were strongly inhibited. On the other hand, channel blockers TEA and NPPB, which inhibited whole-cell currents and RVD, did not inhibit the [Ca2+]i increase. It is concluded tha...Continue Reading

Citations

Apr 2, 2008·Biological & Pharmaceutical Bulletin·Naoko Iida-TanakaHikaru Tanaka
Sep 13, 2003·British Journal of Pharmacology·Feng XuToshihiko Iijima
Aug 11, 2007·Journal of Pharmacological Sciences·Naoko Iida-TanakaHikaru Tanaka
Jul 12, 2002·Journal of Cellular Biochemistry·Clare E YellowleyHenry J Donahue
Feb 27, 2013·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Yu-Mi YangDong Min Shin
Oct 28, 2017·Journal of the American Chemical Society·Takahiro MuraokaKazushi Kinbara

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