Hypotonically loaded rat erythrocytes deliver encapsulated substances into peritoneal macrophages

Journal of Biochemistry
F J AlvarezM C Tejedor

Abstract

Previous work has shown increased uptake of hypotonically loaded rat RBCs by the spleen and liver "in vivo," suggesting that the cells of MPS are involved in their elimination from the circulation. In order to elucidate the mechanism of such elimination, we have undertaken studies on the interaction of such loaded RBCs, in comparison with native RBCs, with peritoneal macrophages. Erythrophagocytosis assays were performed in well plates to which thioglycollate-induced peritoneal macrophages had adhered. Native or loaded 51Cr-RBCs were added under different opsonization conditions to monolayer adherent macrophages, and then the amount of RBCs that were recognized was determined, with separation into adhesion and phagocytosis fractions. Native RBCs are slightly recognized by peritoneal macrophages, about one RBC per macrophage (Mphi). Osmotic treatment of rat RBCs used for encapsulation (independently of the encapsulated substance, 125I-CA or FITC-dextran) produces some modification in the erythrocyte membrane that induces higher recognition of these cells, about three loaded RBCs per macrophage. Consequently, both fluorescent (FITC-Dx) and radioactive (125I-CA) substances previously encapsulated in RBCs were transferred to M(phi)...Continue Reading

Citations

Mar 12, 2004·Journal of Controlled Release : Official Journal of the Controlled Release Society·Carmen Gutiérrez MillánJosé M Lanao
Jan 5, 2002·International Journal of Pharmaceutics·P R Mishra, N K Jain
Mar 3, 2010·Expert Opinion on Drug Delivery·Vladimir R Muzykantov
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Sep 22, 2010·International Journal of Pharmaceutics·Elsa BrionesJosé M Lanao
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May 20, 2015·ACS Biomaterials Science & Engineering·Yixue SuJian Yang
Jun 22, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·T SuzukiZ Honda

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