Hypoxanthine is a checkpoint stress metabolite in colonic epithelial energy modulation and barrier function.

The Journal of Biological Chemistry
J Scott LeeS P Colgan

Abstract

Intestinal epithelial cells form a selectively permeable barrier to protect colon tissues from luminal microbiota and antigens and to mediate nutrient, fluid, and waste flux in the intestinal tract. Dysregulation of the epithelial cell barrier coincides with profound shifts in metabolic energy, especially in the colon, which exists in an energetically depleting state of physiological hypoxia. However, studies that systematically examine energy flux and adenylate metabolism during intestinal epithelial barrier development and restoration after disruption are lacking. Here, to delineate barrier-related energy flux, we developed an HPLC-based profiling method to track changes in energy flux and adenylate metabolites during barrier development and restoration. Cultured epithelia exhibited pooling of phosphocreatine and maintained ATP during barrier development. EDTA-induced epithelial barrier disruption revealed that hypoxanthine levels correlated with barrier resistance. Further studies uncovered that hypoxanthine supplementation improves barrier function and wound healing and that hypoxanthine appears to do so by increasing intracellular ATP, which improved cytoskeletal G- to F-actin polymerization. Hypoxanthine supplementation i...Continue Reading

References

Mar 11, 1975·Journal of Bacteriology·W J Ball, D E Atkinson
Apr 1, 1975·Pediatric Research·O D Saugstad
Oct 1, 1992·The Journal of Clinical Investigation·J B MatthewsJ L Madara
Jan 1, 1991·Annual Review of Cell Biology·A Bretscher
Jan 1, 1987·The Journal of Membrane Biology·J L Madara, J R Pappenheimer
Dec 1, 1971·Journal of Bacteriology·A G ChapmanD E Atkinson
Aug 10, 1973·Biochimica Et Biophysica Acta·A M Mackinnon, D J Deller
May 1, 1983·The Journal of Cell Biology·N HirokawaM S Mooseker
Oct 1, 1981·The Journal of Nutrition·D A Savaiano, A J Clifford
Jan 1, 1994·Gut·G K Grimble
Apr 15, 1996·Archives of Biochemistry and Biophysics·N ErcanF Q Nuttall
Jul 1, 1997·The American Journal of Physiology·O CulicJ Schrader
Oct 31, 1998·Current Opinion in Genetics & Development·G L Semenza
Sep 18, 1999·Digestive Diseases and Sciences·E GaudioR Caprilli
Feb 21, 2002·The Journal of Biological Chemistry·Scott W NelsonHerbert J Fromm
Jun 18, 2002·American Journal of Physiology. Endocrinology and Metabolism·Nacide Ercan-FangFrank Q Nuttall
Aug 3, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Roland H Wenger
Jan 19, 2005·Cell Cycle·Jörn KarhausenSean P Colgan
Dec 6, 2005·Cell·Soichiro YamadaW James Nelson
Mar 31, 2006·American Journal of Physiology. Cell Physiology·Juan C IblaSean P Colgan
Apr 9, 2009·The Journal of Clinical Investigation·Maria MastrogiannakiCarole Peyssonnaux
Jun 23, 2009·Annals of the New York Academy of Sciences·Stefan Koch, Asma Nusrat
Sep 18, 2009·Developmental Cell·Robert H Insall, Laura M Machesky
Oct 27, 2009·Nature Reviews. Immunology·Jerrold R Turner
Apr 7, 2010·Nature Reviews. Gastroenterology & Hepatology·Sean P Colgan, Cormac T Taylor
Jun 29, 2010·The American Journal of Pathology·Andrei I IvanovAsma Nusrat
Sep 10, 2010·BMC Immunology·Izabela RasmussenFrederik Vilhardt
May 3, 2011·Gastroenterology·Louise E Glover, Sean P Colgan
Jun 18, 2011·Science·Jonathan S OakhillBruce E Kemp
Jan 1, 2002·The Arabidopsis Book·Barbara A Moffatt, Hiroshi Ashihara
Apr 24, 2013·Molecular and Cellular Biology·Lydia AschauerPaul Jennings
Nov 20, 2013·Proceedings of the National Academy of Sciences of the United States of America·Louise E GloverSean P Colgan
Oct 11, 2014·PloS One·Ildefonso M De la FuenteLuis Martínez
May 11, 2015·Seminars in Cell & Developmental Biology·Christopher T Capaldo, Asma Nusrat
Jul 17, 2015·American Journal of Physiology. Cell Physiology·Leon ZhengSean P Colgan
May 20, 2016·Annual Review of Pathology·Sean P ColganDouglas J Kominsky

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Citations

May 10, 2019·International Journal of Molecular Sciences·Kamal M YakoubValentina Di Pietro
Jul 30, 2020·Molecular Biology of the Cell·Alexander S DowdellSean P Colgan
Oct 7, 2019·Inflammatory Bowel Diseases·Olga Maria NardoneMarietta Iacucci
May 14, 2020·Proceedings of the National Academy of Sciences of the United States of America·Ruth X WangSean P Colgan
Dec 23, 2020·Journal of Ethnopharmacology·Ye-Ting ZouSong-Lin Li
Feb 13, 2021·International Journal of Environmental Research and Public Health·Xiangyu LiuYumei Han
Feb 14, 2021·Biomolecules·Maria Jose Carranza-NavalAngel Del Marco
Jul 31, 2020·Current Opinion in Physiology·Susana Lechuga, Andrei I Ivanov
Mar 23, 2021·Frontiers in Physiology·Cong YinQingyan Jiang
Apr 8, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Erica E AlexeevSean P Colgan
Dec 19, 2019·Journal of Chemical Information and Modeling·Congcong GongJiaoyan Ren

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