Hypoxia and Inactivity Related Physiological Changes (Constipation, Inflammation) Are Not Reflected at the Level of Gut Metabolites and Butyrate Producing Microbial Community: The PlanHab Study

Frontiers in Physiology
Robert ŠketBlaž Stres

Abstract

We explored the assembly of intestinal microbiota in healthy male participants during the run-in (5 day) and experimental phases [21-day normoxic bed rest (NBR), hypoxic bedrest (HBR)], and hypoxic ambulation (HAmb) in a strictly controlled laboratory environment, balanced fluid, and dietary intakes, controlled circadian rhythm, microbial ambiental burden, and 24/7 medical surveillance. The fraction of inspired O2 (FiO2) and partial pressure of inspired O2 (PiO2) were 0.209 and 133.1 ± 0.3 mmHg for NBR and 0.141 ± 0.004 and 90.0 ± 0.4 mmHg for both hypoxic variants (HBR and HAmb; ~4,000 m simulated altitude), respectively. A number of parameters linked to intestinal transit spanning Bristol Stool Scale, defecation rates, zonulin, α1-antitrypsin, eosinophil derived neurotoxin, bile acids, reducing sugars, short chain fatty acids, total soluble organic carbon, water content, diet composition, and food intake were measured (167 variables). The abundance, structure, and diversity of butyrate producing microbial community were assessed using the two primary bacterial butyrate synthesis pathways, butyryl-CoA: acetate CoA-transferase (but) and butyrate kinase (buk) genes. Inactivity negatively affected fecal consistency and in combina...Continue Reading

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Citations

Sep 14, 2018·American Journal of Physiology. Gastrointestinal and Liver Physiology·J Philip KarlStefan M Pasiakos
Dec 13, 2018·American Journal of Physiology. Gastrointestinal and Liver Physiology·Beatrix PfanzaglErika Jensen-Jarolim
Jul 15, 2020·Experimental Physiology·Angelique Van OmbergenThu Jennifer Ngo-Anh
Sep 28, 2018·Frontiers in Microbiology·J Philip KarlJason W Soares
Jun 6, 2018·Frontiers in Physiology·Tadej DebevecJörn Rittweger
Aug 18, 2018·Oxidative Medicine and Cellular Longevity·Arnold N Onyango

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Methods Mentioned

BETA
urine collection
ELISA
PCR
glycosylation

Clinical Trials Mentioned

NCT02637921

Software Mentioned

mothur
PlanHab
R
FrameBot
MDS
R package vegan

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