Hypoxia and TGF-β1 induced PLOD2 expression improve the migration and invasion of cervical cancer cells by promoting epithelial-to-mesenchymal transition (EMT) and focal adhesion formation

Cancer Cell International
Feifei XuWeijiang Liang

Abstract

Intra-tumoral hypoxia and increases in extracellular level of transforming growth factor β1 (TGF-β1), which are common findings in cancer, are associated with an increased risk of metastasis and mortality. Moreover, metastasis is the leading cause of death of patients with cervical cancer. PLOD2 is an intracellular enzyme required for the biogenesis of collagen and its expression can be induced by hypoxia and TGF-β1. Specifically, PLOD2 is up-regulated in several types of cancer, including cervical cancer, and is associated with cancer metastasis. Thus, in this research, we aimed to investigate the role of PLOD2 in the motility of cervical cancer cells and to show the molecular mechanism underlying this effect. siRNA was used to knockdown PLOD2 in the cervical cancer cell lines HeLa and SiHa. The ability of cells to migrate and invade, their adhesion to type I collagen, and their capacity for epithelial-to-mesenchymal transition (ΕΜΤ) and focal adhesion formation were analyzed. Gene expression changes were validated by qRT-PCR, Western blotting and Immunocytochemistry. The morphological status of cells was examined using phalloidin staining. Differences in PLOD2 expression among patients with cervical cancer were identified by ...Continue Reading

References

Aug 26, 2000·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·S Curran, G I Murray
Oct 13, 2001·International Journal of Radiation Oncology, Biology, Physics·G PitsonR Hill
Nov 1, 2001·Annual Review of Cell and Developmental Biology·M D Sternlicht, Z Werb
Jul 4, 2002·Cell Biology International·Kwonseop KimElizabeth D Hay
May 2, 2003·Nature Reviews. Cancer·Peter Friedl, Katarina Wolf
Jul 5, 2003·Lancet·Steven E Waggoner
Aug 30, 2003·Oncogene·Nicholas C DenkoAmato J Giaccia
Nov 19, 2003·European Journal of Biochemistry·Karl-Heinz HofbauerArmin Kurtz
Dec 31, 2003·Trends in Genetics : TIG·Johanna Myllyharju, Kari I Kivirikko
May 26, 2004·Critical Reviews in Oncology/hematology·G KleinE S J M de Bont
May 24, 2005·Matrix Biology : Journal of the International Society for Matrix Biology·Anne-Marie ZuurmondRuud A Bank
Oct 29, 2005·Journal of Neuropathology and Experimental Neurology·Shumin DongDavid N Louis
Dec 15, 2005·International Journal of Cancer. Journal International Du Cancer·Yick-Fu WongDavid I Smith
Apr 28, 2006·Nature·Janine T ErlerAmato J Giaccia
May 10, 2006·Nature Clinical Practice. Oncology·Patricia J Eifel
Jul 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Muhammad H ZamanPaul Matsudaira
Feb 9, 2007·The Journal of Biological Chemistry·Dexing FangZhimin Lu
Apr 19, 2007·Cancer Metastasis Reviews·Benjamin J MoellerMark W Dewhirst
Sep 11, 2007·Lancet·Mark SchiffmanSholom Wacholder
Feb 26, 2008·Nature Cell Biology·Muh-Hwa YangKou-Juey Wu
Mar 25, 2009·Nature Reviews. Cancer·Don X NguyenJoan Massagué
Jun 3, 2009·The Journal of Clinical Investigation·Raghu Kalluri, Robert A Weinberg
Nov 26, 2009·Cell·Kandice R LeventalValerie M Weaver
Sep 9, 2010·Current Opinion in Cell Biology·Mikala EgebladValerie M Weaver
Feb 8, 2011·CA: a Cancer Journal for Clinicians·Ahmedin JemalDavid Forman
Feb 23, 2011·BMC Cancer·Thangarajan RajkumarGanesharaja Selvaluxmy
Nov 22, 2011·Liver International : Official Journal of the International Association for the Study of the Liver·Takehiro NodaHiroaki Nagano
Jan 24, 2012·Gynecologic Oncology·Vijaya GalicJason D Wright
Dec 1, 2012·Current Opinion in Oncology·Yoko KatsunoRik Derynck
Feb 5, 2013·Molecular Cancer Research : MCR·Daniele M GilkesGregg L Semenza
Aug 3, 2013·Cancer Discovery·T S Karin Eisinger-MathasonM Celeste Simon
May 16, 2014·Nature Reviews. Cancer·Daniele M GilkesDenis Wirtz
Oct 5, 2014·Cancer Letters·Rehana QureshiM A Rizvi

❮ Previous
Next ❯

Citations

Jan 23, 2019·Molecular Carcinogenesis·Mei ZhangYa-Ling Tang
Feb 23, 2019·Journal of Dental Research·T SaitoM Yamauchi
Sep 29, 2020·Cancer Medicine·Anders BerglundJong Y Park
Feb 9, 2020·International Journal of Molecular Sciences·Paola Maroni
Jul 14, 2018·Frontiers in Cell and Developmental Biology·Yifei Qi, Ren Xu
Jul 16, 2019·Cancer Biomarkers : Section a of Disease Markers·Yibo WuZhishan Zhang
Jan 14, 2021·Cell Death & Disease·Chengcheng HeQingyuan Li
May 1, 2021·Journal of Personalized Medicine·Valentina BravatàGiorgio Russo
Jul 15, 2021·BioMed Research International·Guang LiGuobing Liu
Apr 16, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Tiantian QinCong Wang

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
nuclear translocation

Software Mentioned

SPSS Statistics
ImageJ
cBioPortal
Graphpad Prism
Oncomine

Related Concepts

Related Feeds

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.

Biophysics of Adhesion

Alterations in cell adhesion can disrupt important cellular processes and lead to a variety of diseases, including cancer and arthritis. It is also essential for infectious organisms, such as bacteria or viruses, to cause diseases. Understanding the biophysics of cell adhesion can help understand these diseases. Discover the latest research on the biophysics of adhesion here.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.