Hypoxia-challenged MSC-derived exosomes deliver miR-210 to attenuate post-infarction cardiac apoptosis.

Stem Cell Research & Therapy
Hao ChengJianying Ma

Abstract

Myocardial infarction (MI) is a major cause of death worldwide. Although percutaneous coronary intervention and coronary artery bypass grafting can prolong life, cardiac damage persists. In particular, cardiomyocytes have no regenerative capacity. Mesenchymal stem cells (MSCs) are attractive candidates for the treatment of MI. The manner by which MSCs exert a beneficial effect upon injured cells is a source of continued study. After the isolation and identification of exosomes from MSCs, the expression of miR-210 was determined by microarray chip. Subsequently, gain- and loss-function approaches were conducted to detect the role of exosomes and exosomal-miR-210 in cell proliferation and apoptosis of cardiomyocytes, as well as the MI in vivo. Dual-Luciferase Report Gene System was used to demonstrate the target gene of miR-210. We tested the hypothesis that MSC-derived exosomes transfer specific miRNA to protect cardiomyocytes from apoptotic cell death. Interestingly, direct cardiac injection of MSC exosomes reduced infarct size and improved heart function after coronary ligation. In vitro, the MSC exosomes enhanced cardiomyocyte survival to hypoxia. Confirmation of exosome uptake in myocytes was confirmed. Dual-luciferase repor...Continue Reading

References

May 1, 2010·Journal of the American College of Cardiology·Marc CohenMateen Abidi
Jun 7, 2011·Circulation. Cardiovascular Genetics·Yasuhide KuwabaraTakeshi Kimura
Aug 16, 2011·American Journal of Physiology. Heart and Circulatory Physiology·R Kannan MutharasanHossein Ardehali
Aug 25, 2015·Biochimica Et Biophysica Acta·Kobina EssandohGuo-Chang Fan
Jan 27, 2016·Journal of the American Heart Association·Zhiwei ZhangTakayuki Asahara
Aug 23, 2016·Developmental and Comparative Immunology·Chenghua LiWei Xu
Aug 30, 2016·Advanced Healthcare Materials·Krijn R VrijsenJoost P G Sluijter
Sep 30, 2016·World Journal of Stem Cells·Etsu SuzukiHiroaki Nishimatsu
Nov 30, 2016·Circulation Research·Suzanne M EkenLars Maegdefessel
Feb 10, 2017·Journal of Cellular Physiology·Prosenjit PaulSupriyo Chakraborty
Aug 27, 2017·Scientific Reports·G ZaccagniniF Martelli
Oct 3, 2017·Neoplasia : an International Journal for Oncology Research·Xiquan KeStephen J Meltzer
Oct 19, 2017·The Journal of Gene Medicine·Ayman El-Sayed ShafeiAmal S El-Shal
Jan 24, 2018·Nature Immunology·Liang GaoLong Zhang
Apr 6, 2018·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Xiaotang MaCan Chen
Jul 7, 2018·Circulation Research·Eugene Braunwald
Jul 7, 2018·Circulation Research·Kathleen M Broughton, Mark A Sussman
Feb 1, 2019·Circulation·Emelia J BenjaminUNKNOWN American Heart Association Council on Epidemiology and Prevention Statistics Committee and Stroke Statistics Subcommittee
May 10, 2019·Journal of Extracellular Vesicles·Kenneth W WitwerSai Kiang Lim

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Citations

Dec 29, 2020·Frontiers in Pharmacology·Jia LiuJunnan Wang
Mar 23, 2021·Frontiers in Cardiovascular Medicine·Wei Wang, Hao Zheng
Sep 28, 2021·Tissue Engineering. Part B, Reviews·Yanmeng YangZheng Yang
Oct 7, 2021·Journal of Cardiovascular Translational Research·Hui MengMingjing Zhao

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Methods Mentioned

BETA
flow cytometry
electron microscopy
PCR
transfection
Assay
Protein Assay
transmission electron micrography

Software Mentioned

TargetScanHuman
GraphPad Prism
ImageJ

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis