Hypoxia destroys the microstructure of microtubules and causes dysfunction of endothelial cells via the PI3K/Stathmin1 pathway

Cell & Bioscience
Huaming CaoLiang Sheng

Abstract

Endothelial cells (EC) are sensitive to changes in the microenvironment, including hypoxia and ischemia. Disruption of the microtubular network has been reported in cases of ischemia. However, the signaling pathways involved in hypoxia-induced microtubular disruption are unknown. The purpose of this study was to investigate the molecular mechanisms involved in hypoxia-induced microtubular disassembly in human umbilical vein endothelial cells (HUVECs). HUVECs were cultured under normoxic or hypoxic conditions and pretreated with or without colchicine or paclitaxel. The MTT assay, Transwell assay, trans-endothelial permeability assay, and 5-bromo-2'-deoxy-uridine staining were used to test the survival rate, migration, permeability, and proliferation of cells, respectively. Transmission electron microscopy and phalloidin staining were used to observe the microstructure and polymerization of microtubules. The results show that the functions of HUVECs and the microtubular structure were destroyed by hypoxia, but were protected by paclitaxel and a reactive oxygen species (ROS) inhibitor. We further used western blot, a luciferase assay, and co-immunoprecipitation to describe a non-transcription-independent mechanism for PI3K activat...Continue Reading

References

Dec 4, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Anna A BirukovaAlexander D Verin
Apr 14, 2005·Journal of Cellular Physiology·Anna A BirukovaAlexander D Verin
Dec 3, 2005·Cancer Research·Steven J IsakoffJoan S Brugge
Apr 11, 2009·The Journal of Biological Chemistry·Tapas MannaLeslie Wilson
Jan 30, 2010·PloS One·Veronica UliciFrank Beier
Sep 29, 2011·Molecular and Cellular Biochemistry·Sudhakar BaluchamyGovindarajan T Ramesh
Nov 17, 2011·Nature Reviews. Molecular Cell Biology·Carsten Janke, Jeannette Chloë Bulinski
Jul 31, 2012·American Journal of Respiratory Cell and Molecular Biology·Eric KratzerAnna A Birukova
Aug 7, 2012·Genes & Cancer·Bryan L KrockM Celeste Simon
Oct 11, 2013·Molecular Cancer Therapeutics·Anutosh GangulyKamala D Patel
Nov 30, 2013·Free Radical Research·F CervellatiG Valacchi
Jun 7, 2014·Cell·F Jon Kull, Roger D Sloboda
Oct 1, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Simon SchimmackLaura H Tang
May 31, 2015·Vascular Pharmacology·Prasanna Abeyrathna, Yunchao Su
Aug 13, 2015·Nature Reviews. Drug Discovery·Jon O LundbergEddie Weitzberg
Aug 27, 2015·European Heart Journal·Nick TownsendMike Rayner
Jun 25, 2016·The Journal of Biological Chemistry·Zixi WangMin Fang
May 6, 2017·Nature Communications·Edward J MorrisShoukat Dedhar
Jun 24, 2017·The EMBO Journal·Brian W WongPeter Carmeliet

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Citations

May 6, 2020·Micromachines·Miguel Ángel Olmedo-SuárezNobuyuki Futai
Feb 7, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Mohamed ZoughaibTimur I Abdullin
Aug 27, 2021·Metabolites·Alexander Myrka, Leslie Buck

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Methods Mentioned

BETA
chemical treatment
transmission electron microscopy
co-immunoprecipitation assay

Software Mentioned

SPSS

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