Hypoxia does not reduce HLA-G expression on extravillous cytotrophoblasts

Journal of Reproductive Immunology
Takeshi NagamatsuYuji Taketani

Abstract

Placental hypoxia following the immature remodeling of spiral arteries by extravillous cytotrophoblasts (CTs) is focused on the pathogenesis of pre-eclampsia. At the same time, the expression of human leukocyte antigen (HLA)-G is decreased at the protein and mRNA levels in the pre-eclamptic placenta. In view of the potential function of HLA-G in immunological tolerance in the feto-maternal interface, we were much concerned to find whether the lowered expression of HLA-G in the pre-eclamptic placenta is a precursor or the result of placental hypoxia. The effect of oxygen on the expression of membrane-bound (mb) and soluble (s) HLA-G was investigated in primary cultures of extravillous CTs. The undifferentiated CTs isolated from the first-trimester placenta were cultured with different concentrations of oxygen (20%, 8% and 2%). The protein expression of mbHLA-G and of sHLA-G was assessed using flow cytometry, and mRNA expression was analyzed using real-time PCR. Expression of mbHLA-G and of sHLA-G protein was intensified with time in culture regardless of the oxygen concentration, and the expression intensities were synchronized between the 20% and the 2% oxygen concentrations at each time point. The mRNA expressions of mbHLA-G1 ...Continue Reading

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Citations

Nov 17, 2010·Cellular and Molecular Life Sciences : CMLS·Roberta RizzoVera Rebmann
Dec 29, 2015·Human Immunology·Julieta LazarteDiego H Delgado
Jan 19, 2016·Human Immunology·Julieta LazarteDiego Delgado
Jun 11, 2009·Journal of Cellular and Molecular Medicine·Philippe MoreauEdgardo D Carosella
Aug 17, 2006·American Journal of Reproductive Immunology : AJRI·Takeshi NagamatsuYuji Taketani
Apr 13, 2011·American Journal of Reproductive Immunology : AJRI·Morgan R PeltierNazeeh Hanna
Apr 3, 2007·Human Immunology·Gaël MouillotPhilippe Moreau
Apr 28, 2012·Critical Reviews in Clinical Laboratory Sciences·Alvaro GonzálezEstibaliz Alegre
Apr 18, 2014·Journal of Immunology Research·Erick C CastelliEduardo A Donadi
Dec 21, 2017·The Journal of Endocrinology·Ching-Wen ChangMana M Parast
Nov 11, 2005·Human Reproduction Update·Thomas Vauvert F Hviid
Aug 1, 2020·Frontiers in Immunology·Giada Amodio, Silvia Gregori
Aug 7, 2017·Journal of Immunology Research·Marica GarzieraGiuseppe Toffoli
Dec 1, 2020·Journal of Reproductive Immunology·Daniel P JacobsenAnne Cathrine Staff

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