Hypoxia enables B19 erythrovirus to yield abundant infectious progeny in a pluripotent erythroid cell line

Journal of Virological Methods
Perrine Caillet-FauquetRuth Laub

Abstract

B19 may cause mild to severe clinical manifestations. Owing to the remarkable tropism of B19 for red blood cell progenitors, there is a lack of satisfactory cell lines fully permissive for B19. Because the local oxygen pressure may influence viral replication, we used hypoxia to improve the sensitivity of our infectivity assay in order to link B19 DNA detected by PCR to the presence of infectious B19 particles in plasma. Plasma samples and the WHO International Standard for B19 DNA detection by PCR were used to infect the pluripotent human erythroid cell line KU812F under different oxygen pressures. Specific human anti-B19 IgG was found to reduce infectivity. Low oxygen pressure led to higher yields of infectious B19 progeny and to a higher level of viral transcription than observed under normoxia. This sensitive infectivity assay is a promising model for studying B19 biology, identifying neutralising antibodies, and evaluating new virus inactivation methods.

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Citations

Aug 16, 2008·Journal of Virology·Sylvie PilletSusan Wong
Jun 27, 2006·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Perrine Caillet-FauquetRuth Laub
Jun 15, 2013·Journal of Virological Methods·Raphael WolfisbergCarlos Ros
Jun 23, 2015·Future Virology·Yong Luo, Jianming Qiu
Oct 26, 2006·Journal of Leukocyte Biology·Maura PuppoLuigi Varesio
Nov 4, 2016·Clinical Microbiology Reviews·Jianming QiuNeal S Young
Nov 11, 2009·Journal of Physics. Condensed Matter : an Institute of Physics Journal·P GnutekC Rudowicz
Jul 29, 2020·Virology Journal·Esther Shuyi Gan, Eng Eong Ooi
Jun 21, 2018·Frontiers in Cellular and Infection Microbiology·Safder S Ganaie, Jianming Qiu

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