Hypoxia enhances buffalo adipose-derived mesenchymal stem cells proliferation, stemness, and reprogramming into induced pluripotent stem cells

Journal of Cellular Physiology
Yanfei DengSufang Yang

Abstract

Adipose tissue-derived mesenchymal stem cells (ASCs) from livestock are valuable resources for animal reproduction and veterinary therapeutics. Previous studies have shown that hypoxic conditions were beneficial in maintaining the physiological activities of ASCs. However, the effects of hypoxia on buffalo ASCs (bASCs) remain unclear. In this study, the effects of hypoxia on proliferation, stemness, and reprogramming into induced pluripotent stem cells (iPSCs) of bASCs were examined. The results showed that the hypoxic culture conditions (5% oxygen) enhanced the proliferation and colony formation of bASCs. The expression levels of proliferation-related genes, and secretion of basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF) were significantly enhanced in hypoxia. Hypoxic culture conditions activated hypoxia-inducible factor-1α (HIF-1α), thereby contributing to the secretion of bFGF and VEGF, which in turn enhanced the expression of HIF-1α and promoted the proliferation of bASCs. Furthermore, in hypoxic culture conditions, bASCs exhibited the main characteristics of mesenchymal stem cells, and the expression levels of the pluripotent markers OCT4, NANOG, C-MYC, and the differentiation capacity ...Continue Reading

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Citations

Oct 2, 2020·In Vitro Cellular & Developmental Biology. Animal·Jun ZhangFenghua Lu
Oct 8, 2020·Stem Cell Reviews and Reports·Joanna BukowskaBarbara Gawrońska-Kozak
Aug 5, 2020·Reproduction in Domestic Animals = Zuchthygiene·Jun ZhangFenghua Lu
Aug 28, 2021·Cells·Miriam Di MattiaBarbara Barboni

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