Hypoxia enhances colony formation and proliferation but inhibits differentiation of human dental pulp cells

Archives of Oral Biology
Kazuki IidaK Tezuka

Abstract

The hypoxia condition was expected to be suitable for the establishment and maintenance of human dental pulp cells (hDPCs), because they reside in a low-oxygen environment in vivo. Therefore, we presently examined the effects of hypoxia on the proliferation and differentiation of hDPCs in vitro. hDPCs grown under 3% O(2) showed a significantly higher proliferation rate than those under 21% O(2). Then, we prepared hypoxic cultures of hDPCs from older patients' teeth having inflammation and succeeded in recovering and expanding a small number of hDPCs. These cells were confirmed to have capability for osteo/odontogenic differentiation. Hypoxia suppressed the osteo/odontogenic differentiation of hDPCs in vitro and increased the number of cells expressing STRO-1, an early mesenchymal stem cell marker. This simple method will increase the possibility to obtain living hDPCs from damaged and/or aged tissues, from which it is ordinarily difficult to isolate living stem cells with differentiation capability.

References

Oct 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·E L Schneider, Y Mitsui
Jul 31, 2002·Journal of Dental Research·S GronthosS Shi
Sep 28, 2002·Journal of Dental Research·C S YoungP C Yelick
Apr 4, 2003·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Songtao Shi, Stan Gronthos
Jul 31, 2003·European Journal of Oral Sciences·Kei AmemiyaTakashi Inoue
Aug 26, 2006·Journal of Dental Research·Y UenoT Nishihara
Feb 27, 2007·Nature Methods·Kazuhisa NakaoTakashi Tsuji
Aug 28, 2007·Journal of Dental Research·Y FukuyamaK Nakagawa
Jun 21, 2008·Differentiation; Research in Biological Diversity·Hideki AgataMinoru Ueda
Jun 25, 2008·Journal of Dental Research·T TakedaK Tezuka
Jun 2, 2009·Journal of Endodontics·Jaruma Beau SakdeePeter V Hauschka
Jun 6, 2009·Journal of Dental Research·S A Hacking, A Khademhosseini
Feb 23, 2010·Journal of Endodontics·Jinming WangQimei Gong

❮ Previous
Next ❯

Citations

Jul 28, 2013·Fiziologiia cheloveka·E R andreeva, L B Buravkova
Aug 26, 2014·Journal of Endodontics·Julie VanackerAnne des Rieux
Jul 19, 2014·Mitochondrion·L B BuravkovaB Zhivotovsky
Mar 17, 2012·Biotechnology and Bioengineering·Andrew B YeattsJohn P Fisher
Jul 4, 2012·International Endodontic Journal·L O PereiraR B Azevedo
Apr 6, 2016·Archives of Oral Biology·Stefanie Bressan WerleLuciano Casagrande
Jun 8, 2013·Biochimica Et Biophysica Acta·L B BuravkovaV Gogvadze
Dec 19, 2014·PloS One·Tomoko Takeda-KawaguchiKen-Ichi Tezuka
Oct 1, 2016·Stem Cells International·Jessica RatajczakPetra Hilkens
Aug 22, 2013·Journal of Dental Research·K IidaK Tezuka
Apr 8, 2018·Clinical Oral Investigations·Stefanie Bressan WerleLuciano Casagrande
Jun 19, 2018·The Journal of International Medical Research·Beatriz Hernández-MonjarazVíctor Manuel Mendoza-Núñez
Jun 23, 2018·International Journal of Molecular Sciences·Fukushima TatsuhiroSatomura Kazuhito
Feb 8, 2018·Brazilian Oral Research·Benício Daniela Ferreira AraújoBezerra Ana Cristina Barreto
Jul 15, 2020·Biology·Shinichiro YoshidaHidefumi Maeda
Dec 23, 2017·Frontiers in Physiology·Beatriz A Rodas-JuncoGeovanny I Nic-Can
Aug 30, 2020·International Journal of Molecular Sciences·Anna Labedz-MaslowskaEwa Zuba-Surma
May 18, 2018·Frontiers in Bioengineering and Biotechnology·Mona K Marei, Rania M El Backly
Sep 7, 2018·Journal of Bone and Mineral Metabolism·Ken SugiyamaKen-Ichi Tezuka

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.