Hypoxia enhances MUC1 expression in a lung adenocarcinoma cell line

Biochemical and Biophysical Research Communications
Yuji MikamiHiroshi Katsuki

Abstract

Expression of a transmembrane mucin MUC1 is emphasized in most cases of carcinoma. High expression of MUC1 is closely associated with cancer progression and metastasis, leading to poor prognosis. However, little is known about how MUC1 is overexpressed in malignant tumor. In this study, we demonstrated that: (1) Hypoxia, a typical feature of malignant tumor, enhanced the expression of MUC1 mRNA and protein in a human lung adenocarcinoma cell line; (2) the hypoxia-induced increase in MUC1 mRNA was mediated by the transcriptional activity of MUC1 promoter, but not mRNA stability. Moreover; (3) CoCl(2), an inducer of Hypoxia Inducible Factor (HIF)-1alpha, increased the expression of MUC1 mRNA; and (4) HIF-1alpha-targeted siRNA but not its control siRNA decreased hypoxia-induced MUC1 mRNA. These data suggest that hypoxia enhances the expression of MUC1 through the transcriptional regulation by HIF-1alpha in a human lung epithelial cell line.

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Citations

Oct 4, 2011·Pathology Oncology Research : POR·Kyoichi KairaNobuyuki Yamamoto
Dec 10, 2009·Cancer Research·Mohit Sachdeva, Yin-Yuan Mo
May 28, 2011·Cellular and Molecular Life Sciences : CMLS·Jingqi YanHonglian Shi
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Jan 12, 2011·Biochemical and Biophysical Research Communications·Akinori HisatsuneHiroshi Katsuki
Jan 15, 2014·Biochimica Et Biophysica Acta·Kamiya Mehla, Pankaj K Singh
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May 3, 2019·Oncogene·Nadine RohwerThorsten Cramer
Nov 24, 2011·International Journal of Surgical Pathology·Kyoichi KairaNobuyuki Yamamoto

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