Hypoxia impairs adaptation of skeletal muscle protein turnover- and AMPK signaling during fasting-induced muscle atrophy

PloS One
Chiel C de TheijeRamon C J Langen

Abstract

Hypoxemia in humans may occur during high altitude mountaineering and in patients suffering from ventilatory insufficiencies such as cardiovascular- or respiratory disease including Chronic Obstructive Pulmonary Disease (COPD). In these conditions, hypoxemia has been correlated to reduced appetite and decreased food intake. Since hypoxemia and reduced food intake intersect in various physiological and pathological conditions and both induce loss of muscle mass, we investigated whether hypoxia aggravates fasting-induced skeletal muscle atrophy and evaluated underlying protein turnover signaling. Mice were kept under hypoxic (8% oxygen) or normoxic conditions (21% oxygen), or were pair-fed to the hypoxia group for 12 days. Following an additional 24 hours of fasting, muscle weight and protein turnover signaling were assessed in the gastrocnemius muscle by RT-qPCR and Western blotting. Loss of gastrocnemius muscle mass in response to fasting in the hypoxic group was increased compared to the normoxic group, but not to the pair-fed normoxic control group. Conversely, the fasting-induced increase in poly-ubiquitin conjugation, and expression of the ubiquitin 26S-proteasome E3 ligases, autophagy-lysosomal degradation-related mRNA tra...Continue Reading

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Citations

Apr 3, 2019·American Journal of Physiology. Endocrinology and Metabolism·Erin Faught, Mathilakath M Vijayan
Apr 5, 2020·International Journal of Molecular Sciences·Franziska Dengler
Nov 3, 2020·Expert Review of Respiratory Medicine·Harry R GoskerSami O Simons
Jun 26, 2021·Frontiers in Cell and Developmental Biology·Qiang ZhaoMing-Hui Zou

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Methods Mentioned

BETA
PCR

Software Mentioned

LinRegPCR
Primer Express
SPSS

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