Hypoxia impedes hypertrophic chondrogenesis of human multipotent stromal cells

Tissue Engineering. Part a
Debby GawlittaWouter J A Dhert

Abstract

Within the field of bone tissue engineering, the endochondral approach to forming bone substitutes represents a novel concept, where cartilage will undergo hypertrophic differentiation before its conversion into bone. For this purpose, clinically relevant multipotent stromal cells (MSCs), MSCs, can be differentiated into the chondrogenic lineage before stimulating hypertrophy. Controversy exists in literature on the oxygen tensions naturally present during this transition in, for example, the growth plate. Therefore, the present study focused on the effects of different oxygen tensions on the progression of the hypertrophic differentiation of MSCs. Bone marrow-derived MSCs of four human donors were expanded, and differentiation was induced in aggregate cultures. Normoxic (20% oxygen) and hypoxic (5%) conditions were imposed on the cultures in chondrogenic or hypertrophic differentiation media. After 4 weeks, the cultures were histologically examined and by real-time polymerase chain reaction. Morphological assessment showed the chondrogenic differentiation of cultures from all donors under normoxic chondrogenic conditions. In addition, hypertrophic differentiation was observed in cultures derived from all but one donor. The dep...Continue Reading

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Citations

Nov 30, 2012·Current Opinion in Rheumatology·Brian O Diekman, Farshid Guilak
Nov 22, 2014·Journal of Tissue Engineering and Regenerative Medicine·Nicole GeorgiMarcel Karperien
Jun 21, 2014·International Journal of Endocrinology·Alice M C LeeCory J Xian
Apr 20, 2016·Stem Cell Research & Therapy·Jonathan C Bernhard, Gordana Vunjak-Novakovic
Oct 16, 2014·Tissue Engineering. Part a·Debby GawlittaWouter J A Dhert
Apr 30, 2016·Connective Tissue Research·Thomas J KeanJames E Dennis
Nov 26, 2015·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Adam O'Reilly, Daniel J Kelly
Jun 4, 2016·Journal of Tissue Engineering and Regenerative Medicine·Xing ZhaoMark A Randolph
Jun 28, 2016·Biotechnology and Bioengineering·Guifang GaoXiaofeng Cui
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Oct 7, 2014·Macromolecular Bioscience·Nandana BhardwajBiman B Mandal
Oct 9, 2018·Expert Opinion on Biological Therapy·Jianyu ZouYongchang Yao
Nov 7, 2018·Tissue Engineering. Part C, Methods·Michella H HagmeijerDaniel B F Saris
Jan 31, 2016·Biomechanics and Modeling in Mechanobiology·Adam OReillyDaniel J Kelly
Aug 1, 2020·Frontiers in Bioengineering and Biotechnology·Alessia LongoniDebby Gawlitta
May 22, 2019·Tissue Engineering. Part C, Methods·Koen DijkstraDaniël B F Saris
Jan 27, 2019·International Journal of Molecular Sciences·Girish PattappaPeter Angele
Oct 22, 2016·Stem Cell Research & Therapy·Devon E AndersonBrian Johnstone

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Methods Mentioned

BETA
biopsies
fluorescence-activated cell sorting
light microscopy
ELISA
PCR

Software Mentioned

SPSS

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