Hypoxia-induced down-regulation of microRNA-34a promotes EMT by targeting the Notch signaling pathway in tubular epithelial cells.

PloS One
Rui DuShiren Sun

Abstract

Hypoxia-induced renal tubular cell epithelial-mesenchymal transition (EMT) is an important event leading to renal fibrosis. MicroRNAs (miRNAs) are small non-coding RNA molecules that bind to their mRNA targets, thereby leading to translational repression. The role of miRNA in hypoxia-induced EMT is largely unknown. miRNA profiling was performed for the identification of differentially expressed miRNAs in HK-2 cells under normal and low oxygen, and the results were then verified by quantitative real time RT-PCR (qRT-PCR). The function of miRNAs in hypoxia-induced renal tubular cell EMT was assessed by the transfection of specific miRNA inhibitors and mimics. Luciferase reporter gene assays and western blot analysis were performed to validate the target genes of miR-34a. siRNA against Jagged1 was designed to investigate the role of the miR-34a-Notch pathway in hypoxia induced renal tubular cell EMT. miRNA-34a was identified as being downregulated in hypoxic renal tubular epithelial cells. Inhibition of miR-34a expression in HK-2 cells, which highly express endogenous miR-34a, promoted a mesenchymal phenotype accompanied by reduced expression of the epithelial marker Z0-1, E-cadherin and increased expression of the mesenchymal mar...Continue Reading

References

Sep 28, 1995·Nature·S JarriaultA Israel
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jun 1, 2002·Journal of the American Society of Nephrology : JASN·Jeremiah MorrisseySaulo Klahr
Sep 26, 2003·The American Journal of Pathology·Tsutomu ImaiIkuo Konishi
Jan 1, 2004·Genes & Development·Luika A TimmermanJosé Luis de la Pompa
May 5, 2004·The Journal of Biological Chemistry·Indranil DasJan Kitajewski
Sep 24, 2004·Current Opinion in Genetics & Development·Tom Kadesch
Nov 1, 2005·Developmental Cell·Maria V GustafssonMaria Bondesson
Nov 18, 2005·Journal of the American Society of Nephrology : JASN·Masaomi Nangaku
Nov 25, 2005·The New England Journal of Medicine·Edward F AttiyehUNKNOWN Children's Oncology Group
Nov 30, 2005·Nucleic Acids Research·Cindy VandewalleGeert Berx
Feb 3, 2006·Molecular Biology of the Cell·Wang ShengBurton B Yang
Apr 28, 2006·Nature·Janine T ErlerAmato J Giaccia
Aug 22, 2006·Nature Reviews. Molecular Cell Biology·Sarah J Bray
Oct 25, 2006·Molecular and Cellular Biology·Andrew J EvansMichael Ohh
Dec 30, 2006·Molecular and Cellular Biology·Ritu KulshreshthaMircea Ivan
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Mitsuo KatoRama Natarajan
Jun 22, 2007·Cell Cycle·Ritu KulshreshthaMircea Ivan
Sep 19, 2007·Proceedings of the National Academy of Sciences of the United States of America·Hiroshi TazawaHitoshi Nakagama
Nov 27, 2007·The Journal of Clinical Investigation·Debra F HigginsVolker H Haase
Dec 15, 2007·The Journal of Clinical Investigation·Jonas SjölundHåkan Axelson
Feb 27, 2008·Proceedings of the National Academy of Sciences of the United States of America·Xiaofeng ZhengLorenz Poellinger
Mar 4, 2008·Nature Medicine·Thiruvur NiranjanKatalin Susztak
Apr 18, 2008·Cell Cycle·Debra F HigginsVolker H Haase
Oct 7, 2008·TheScientificWorldJournal·Emily L PatersonGregory J Goodall
Oct 11, 2008·Nature Reviews. Cancer·Bradly G Wouters, Marianne Koritzinsky
Oct 18, 2008·The Journal of Clinical Investigation·Barbara BedogniMarianne Broome Powell
Mar 4, 2009·Nature Cell Biology·Julia WinterSven Diederichs
May 23, 2009·Cell Death and Differentiation·H Hermeking
Oct 23, 2009·Annals of the New York Academy of Sciences·Volker H Haase
Nov 11, 2009·Biochemical and Biophysical Research Communications·Rui DuDaiming Fan
Nov 17, 2009·American Journal of Hypertension·Gang WangCheuk-Chun Szeto
Jan 9, 2010·Journal of the American Society of Nephrology : JASN·Aleksandra KrupaDonald Fraser

❮ Previous
Next ❯

Citations

May 13, 2014·Journal of Molecular Cell Biology·Matjaz RokavecHeiko Hermeking
Jul 18, 2014·Cell Death & Disease·X J LiJ H Tang
Sep 24, 2013·Antioxidants & Redox Signaling·Simona GrecoFabio Martelli
Oct 12, 2013·Antioxidants & Redox Signaling·Harriet E GeeMircea Ivan
Sep 25, 2014·Science Signaling·David M Gonzalez, Damian Medici
Dec 3, 2014·Vascular Pharmacology·Bahman RostamaLucy Liaw
Apr 12, 2013·Archivum Immunologiae Et Therapiae Experimentalis·Agata MatejukClaudine Kieda
Feb 23, 2013·Clinical and Experimental Nephrology·Jianhua HeKeizo Kanasaki
Dec 18, 2012·Journal of Biomedical Science·Ya-Ping Tsai, Kou-Juey Wu
Mar 19, 2016·Stem Cells International·Ching-Wen LinPan-Chyr Yang
Apr 25, 2014·Expert Opinion on Therapeutic Targets·Kevin R GinnebaughFazlul H Sarkar
Oct 17, 2015·International Journal of Molecular Sciences·Rui PengQin Zhou
Oct 16, 2015·Molecular Medicine Reports·Tianming LiuYaguang Weng
Oct 16, 2012·Seminars in Cell & Developmental Biology·Chandan K Sen, Sashwati Roy
Dec 10, 2015·The International Journal of Biochemistry & Cell Biology·Haifeng LiuHongjian Li
Jul 19, 2013·The EMBO Journal·Karine LefortGian Paolo Dotto
Sep 14, 2012·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Ana Laura De Lella EzcurraPablo Wappner
Mar 11, 2015·Reviews in Medical Virology·Jiezhong Chen
Nov 11, 2015·Journal of Cellular Physiology·Sabrina De CarolisMassimiliano Bonafé
Aug 25, 2012·Molecular Cancer·Zhengang PengHuey-Jen L Lin
Sep 15, 2012·Journal of Experimental & Clinical Cancer Research : CR·Nan YouKaishan Tao
Nov 22, 2015·Briefings in Functional Genomics·Hani Choudhry, David R Mole
Jun 27, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Ravindresh Chhabra, Neeru Saini
Mar 15, 2013·The Biochemical Journal·Shanshan LiDaling Zhu
Oct 18, 2014·Expert Opinion on Therapeutic Targets·Ying HuangDai-fu Zhang
May 29, 2013·Free Radical Biology & Medicine·Shriram NallamshettyJoseph Loscalzo
Oct 2, 2013·Epigenetics : Official Journal of the DNA Methylation Society·Ludivine DoridotDaniel Vaiman
Sep 21, 2016·Chinese Medical Journal·Cui YangShao-Jun Chen
May 6, 2014·Journal of Molecular and Cellular Cardiology·Bin-Bin ChengZong-Lai Jiang
Dec 6, 2016·European Journal of Pharmacology·Xiao-Zhou ZouZheng Zhang
Jan 18, 2017·Journal of Cellular and Molecular Medicine·Minna LiuQi Qian
Dec 25, 2016·Critical Reviews in Oncology/hematology·Jerome Lacombe, Frederic Zenhausern

❮ Previous
Next ❯

Methods Mentioned

BETA
biopsy
biopsies
PCR
transfections
transfection
protein assay

Software Mentioned

Genepix Pro
Excel
BLASTing
SPSS12
Bio Image IQ
Quantity One
SPSS

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

B-Cell Leukemia (Keystone)

B-cell leukemia includes various types of lymphoid leukemia that affect B cells. Here is the latest research on B-cell leukemia.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.