Hypoxia-Induced Epithelial-Mesenchymal Transition Is Involved in Bleomycin-Induced Lung Fibrosis

BioMed Research International
Liang GuoRong Zhu

Abstract

Pulmonary fibrosis is a severe disease that contributes to the morbidity and mortality of a number of lung diseases. However, the molecular and cellular mechanisms leading to lung fibrosis are poorly understood. This study investigated the roles of epithelial-mesenchymal transition (EMT) and the associated molecular mechanisms in bleomycin-induced lung fibrosis. The bleomycin-induced fibrosis animal model was established by intratracheal injection of a single dose of bleomycin. Protein expression was measured by Western blot, immunohistochemistry, and immunofluorescence. Typical lesions of lung fibrosis were observed 1 week after bleomycin injection. A progressive increase in MMP-2, S100A4, α-SMA, HIF-1α, ZEB1, CD44, phospho-p44/42 (p-p44/42), and phospho-p38 MAPK (p-p38) protein levels as well as activation of EMT was observed in the lung tissues of bleomycin mice. Hypoxia increased HIF-1α and ZEB1 expression and activated EMT in H358 cells. Also, continuous incubation of cells under mild hypoxic conditions increased CD44, p-p44/42, and p-p38 protein levels in H358 cells, which correlated with the increase in S100A4 expression. In conclusion, bleomycin induces progressive lung fibrosis, which may be associated with activation ...Continue Reading

References

Dec 9, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·William D HardieThomas R Korfhagen
Aug 23, 2006·Proceedings of the National Academy of Sciences of the United States of America·Kevin K KimHarold A Chapman
Jul 17, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Brigham C Willis, Zea Borok
Nov 28, 2007·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Rubin M TuderIwona Fijalkowska
Dec 29, 2007·The Journal of Pathology·T A Wynn
May 15, 2008·BioTechniques·Ralf-Harto HübnerBurkhard Bewig
Feb 18, 2009·Seminars in Respiratory and Critical Care Medicine·Robert A C CohenFrancis H Y Green
Jun 13, 2009·American Journal of Respiratory Cell and Molecular Biology·Yongju LuYon Rojanasakul
Jun 27, 2009·American Journal of Respiratory and Critical Care Medicine·Harikrishna TanjoreWilliam E Lawson
Oct 6, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Guofei ZhouJacob I Sznajder
Apr 17, 2010·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Sarah ParkJie-Young Song
Jun 22, 2010·American Journal of Physiology. Lung Cellular and Molecular Physiology·Amber L DegryseWilliam E Lawson
Aug 10, 2010·Clinics in Chest Medicine·James C Lee, Vivek N Ahya
Oct 5, 2010·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·John Yarnold, Marie-Catherine Vozenin Brotons
Mar 8, 2012·Molecular Therapy : the Journal of the American Society of Gene Therapy·Allison N LauDaniel J Weiss
Dec 12, 2012·Biochimica Et Biophysica Acta·Andrew E Vaughan, Harold A Chapman
Aug 6, 2013·Current Drug Targets·Nian-Hua DingLun-Quan Sun
Sep 7, 2013·Chest·Nobuaki Hirota, James G Martin

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Citations

Oct 19, 2017·International Journal of Molecular Sciences·Evangelia PardaliPeter Ten Dijke
Oct 14, 2017·Bioscience Reports·Michael Kasper, Kathrin Barth
Jan 8, 2021·Frontiers in Cell and Developmental Biology·Jacopo Di GregorioVincenzo Flati

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